Rabbit anti Human MAGE3 Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function: Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Recombinant MAGE3 Antibody (Ab114005) - Western Blot All lanes: Recombinant MAGE3 Antibody (Ab114005) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 35 kDa Observed band size: 39 kDa Exposure time: 12.2 s
Recombinant MAGE3 Antibody (Ab114005) - Western Blot All lanes: Recombinant MAGE3 Antibody (Ab114005) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 35 kDa Observed band size: 39 kDa Exposure time: 12.2 s