E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as an ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways.
宿主种属
兔(Rabbit)
特异性
MDM2
种属反应性
人(Human),猴(Monkey)
免疫原
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MDM2.
阳性对照
WB: SK-OV-3 cell lysate. IHC: Human lung cancer tissue. IF/ICC: Jurkat cells.
MDM2 Antibody(Ab114718) - Western Blot All lanes: MDM2 Antibody(Ab114718) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 53 kDa Exposure time: 100.0 s
MDM2 Antibody(Ab114718) - IHC Immunohistochemical analysis of MDM2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MDM2 Antibody(Ab114718) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
MDM2 Antibody(Ab114718) - IF/ICC Immunofluorescent analysis of MDM2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with MDM2 Antibody(Ab114718) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
ZJ25F0319249
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