ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human MGEA5/OGA Antibody, Recombinant, could be used for WB, IF/ICC and so on.
Application:
WB: 1/1000-1/5000
IF/ICC: 1/50-1/200
Protein Function:
Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Recombinant MGEA5/OGA Antibody (Ab115221) - Western Blot
All lanes: Recombinant MGEA5/OGA Antibody (Ab115221) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 103 kDa
Observed band size: 119 kDa
Exposure time: 1.0 s
Recombinant MGEA5/OGA Antibody (Ab115221) - IF/ICC
Immunofluorescent analysis of HeLa, using Recombinant MGEA5/OGA Antibody (Ab115221) at 1/50 dilution.
Recombinant MGEA5/OGA Antibody (Ab115221) - IF/ICC
Immunofluorescent analysis of HeLa, using Recombinant MGEA5/OGA Antibody (Ab115221) at 1/50 dilution.
1/50 - 1/200
1/1000 - 1/2000
1/1000 - 1/5000
1/50-1/200
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0708422 |
分析证书 | Ab115221 |
| ZJ24F0708423 |
分析证书 | Ab115221 |
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