Rabbit anti Human MICB Antibody, Polyclonal, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/2000 IHC: 1/50-1/200
Protein Function:
Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
规格
产品名称
MICB Antibody
别名
Anti-MICB抗体 | MICB抗体
英文别名
MHC class I chain-related protein B | MHC class I mic-B antigen | MHC class I polypeptide-related sequence B | MICB | MIC-B | PERB11.2MHC class I-like molecule PERB11.2-IMX | stress inducible class I homolog | MHC class I chain related protein B antibody
规格或纯度
ExactAb™, Validated, 1.0 mg/mL
宿主种属
兔(Rabbit)
特异性
MICB
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human MICB
阳性对照
WB: Jurkat, Raji, Ramos, K562, U-2 OS, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
MICB Antibody (Ab115326) - Western Blot All lanes: MICB Antibody (Ab115326) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 43 kDa Observed band size: 42 kDa Exposure time: 73.4 s
MICB Antibody (Ab115326) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MICB Antibody (Ab115326). Tris-EDTA, pH9.0 was used for antigen retrieval. Antibody was diluted at 1/200 (4℃ overnight. Secondary antibody was diluted at 1/200 (room temperature, 45 min).
MICB Antibody (Ab115326) - IF/ICC IF analysis of MICB Antibody (Ab115326) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MICB Antibody (Ab115326) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).