Recombinant MLKL Antibody (Ab115571) - Western Blot All lanes: Recombinant MLKL Antibody (Ab115571) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 21.0 s
Recombinant MLKL Antibody (Ab115571) - IF/ICC IF analysis of Recombinant MLKL Antibody (Ab115571) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MLKL Antibody (Ab115571) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).