Mouse anti Human MMP2 Antibody, Monoclonal (C1), could be used for WB, IHC and so on.
规格
产品名称
MMP2 Mouse mAb
别名
基质金属蛋白酶2抗体 | 基质金属蛋白酶2单克隆抗体 | MMP2抗体 | MMP2单克隆抗体
英文别名
72 kDa gelatinase | CLG4 | CLG4A72 kDa type IV collagenase | collagenase type IV-A | EC 3.4.24 | EC 3.4.24.24 | Gelatinase A | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) | matrix metalloproteinase 2 (gelatinase A,
规格或纯度
无载体, ExactAb™, 已验证, 高性能, 见COA
生化机理
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly--Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
宿主种属
小鼠(Mouse)
特异性
MMP2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant MMP2 expressed in E.coli (AA 110-660)
阳性对照
WB: RAW 264.7, C6, HUVEC, A431, SK-N-SH and MCF7 cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
MMP2 Mouse mAb (Ab115657) - Western Blot All lanes: MMP2 Mouse mAb (Ab115657) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 29.4 s
MMP2 Mouse mAb (Ab115657) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 20 µg/mL MMP2 Mouse mAb (Ab115657) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
MMP2 Mouse mAb (Ab115657) - IF/ICC IF analysis of MMP2 Mouse mAb (Ab115657) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MMP2 Mouse mAb (Ab115657) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F1011778
危险品化学品经营许可证（带存储）