The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
宿主种属
兔(Rabbit)
特异性
MUC1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Mucin 1 (AA 1200-1255).
阳性对照
WB: Mouse stomach, Rat lung, MCF7, HeLa and A431 lysates. IHC: Human ovarian cancer tissue. IF/ICC: HeLa cells. IP: HeLa cells.
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
图片
Recombinant MUC1 Antibody (Ab116371) - Western Blot All lanes: Recombinant MUC1 Antibody (Ab116371) at 1/1000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 122 kDa Observed band size: 20, 23 kDa MUC1 is a type I transmembrane glycoprotein widely expressed in various epithelial tissues. Following translation, it undergoes cleavage in the endoplasmic reticulum to generate an extracellular N-terminal subunit (120 - 200 kDa) and a cytoplasmic C-terminal subunit (23 - 30 kDa) (PMID: 15270554). Recombinant MUC1 Antibody (Ab116371) was raised against the C-terminal region of human MUC1, thus it recognizes the 23 - 30 kDa cytoplasmic MUC1. Exposure time: 17.8 s
Recombinant MUC1 Antibody (Ab116371) - Western Blot All lanes: Recombinant MUC1 Antibody (Ab116371) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 122 kDa Observed band size: 21- 25 kDa MUC1 is a type I transmembrane glycoprotein widely expressed in various epithelial tissues. Following translation, it undergoes cleavage in the endoplasmic reticulum to generate an extracellular N-terminal subunit (120 - 200 kDa) and a cytoplasmic C-terminal subunit (23 - 30 kDa) (PMID: 15270554). Recombinant MUC1 Antibody (Ab116371) was raised against the C-terminal region of human MUC1, thus it recognizes the 23 - kDa cytoplasmic MUC1. Exposure time: 21.0 s
Recombinant MUC1 Antibody (Ab116371) - Western Blot All lanes: Recombinant MUC1 Antibody (Ab116371) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution Predicted band size: 122 kDa Observed band size: 25 kDa MUC1 is a type I transmembrane glycoprotein widely expressed in various epithelial tissues. Following translation, it undergoes cleavage in the endoplasmic reticulum to generate an extracellular N-terminal subunit (120 - 200 kDa) and a cytoplasmic C-terminal subunit (23 - 30 kDa) (PMID: 15270554). Recombinant MUC1 Antibody (Ab116371) was raised against the C-terminal region of human MUC1, thus it recognizes the 23 - 30 kDa cytoplasmic MUC1. Exposure time: 41.3 s
Recombinant MUC1 Antibody (Ab116371) - IP Immunoprecipitate (IP) analysis using the Recombinant MUC1 Antibody (Ab116371) at 1/50 dilution. (WB with Recombinant MUC1 Antibody (Ab116371) at 1/6000 dilution)
Recombinant MUC1 Antibody (Ab116371) - IF/ICC Immunofluorescent analysis of HeLa cells, using the Recombinant MUC1 Antibody (Ab116371) at 1/50 dilution.
Recombinant MUC1 Antibody (Ab116371) - IHC Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Recombinant MUC1 Antibody (Ab116371).
ZJ25F0521426
危险品化学品经营许可证（带存储）