ExactAb™,已验证,敲低验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human p60 katanin Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/2000
IF/ICC: 1/200
Protein Function
Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays, such as during disassembly of interphase microtubules at the G2-M transition. May also be required for microtubule release from the centrosome after nucleation. In mitotic spindles this could allow depolymerization of the microtubule end proximal to the centrosome, and subsequent poleward microtubule flux. In neurons, microtubule release within the cell body may allow their subsequent transport into neuronal processes by microtubule dependent motor proteins. This transport is required for axonal growth.
Recombinant p60 katanin Antibody (Ab119891) - Western Blot
All lanes: Recombinant p60 katanin Antibody (Ab119891) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 126.0 s
Recombinant p60 katanin Antibody (Ab119891) - Western Blot
All lanes: Recombinant p60 katanin Antibody (Ab119891) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 10.0 s
Recombinant p60 katanin Antibody (Ab119891) - Western Blot
All lanes use Recombinant p60 katanin Antibody (Ab119891) at 1/1000 dilution for 1 hour at room temperature.
Recombinant p60 katanin Antibody (Ab119891) - IF/ICC
IF analysis of Recombinant p60 katanin Antibody (Ab119891) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant p60 katanin Antibody (Ab119891) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
1/200
1/500-1/2000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0708780 |
分析证书 | Ab119891 |
| ZJ24F0708781 |
分析证书 | Ab119891 |
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