Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
Post-translational: Autophosphorylated. N-glycosylated.
宿主种属
兔(Rabbit)
特异性
EIF2AK3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human PERK (AA 873-1116)
阳性对照
WB: K562, HeLa, MCF7, HEK293, NIH/3T3 and C6 cell lysates.
Recombinant PERK Antibody (Ab121304) - Western Blot All lanes: Recombinant PERK Antibody (Ab121304) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 141 kDa Exposure time: 20.8 s