Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
宿主种属
兔(Rabbit)
特异性
PHD2/prolyl hydroxylase
种属反应性
人(Human),小鼠(Mouse)
免疫原
A synthetic peptide derived from human PHD2/prolyl hydroxylase (AA 1-51)
Recombinant PHD2/prolyl hydroxylase Antibody (Ab121566) - Western Blot All lanes: Recombinant PHD2/prolyl hydroxylase Antibody (Ab121566) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 47 kDa Exposure time: 256.6 s