Rabbit anti Human PODXL Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Recombinant PODXL Antibody (Ab122517) - Western Blot All lanes: Recombinant PODXL Antibody (Ab122517) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 59 kDa Observed band size: 164,183 kDa Exposure time: 76.5 s
Recombinant PODXL Antibody (Ab122517) - Western Blot All lanes: Recombinant PODXL Antibody (Ab122517) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 59 kDa Observed band size: 164, 183 kDa Exposure time: 28.3 s