Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
宿主种属
兔(Rabbit)
特异性
SMAD2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Smad2 (AA 230-270)
阳性对照
WB: HeLa, Jurkat, Hep G2, MCF7, SH-SY5Y, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Recombinant Smad2 Antibody (Ab128194) - Western Blot All lanes: Recombinant Smad2 Antibody (Ab128194) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 58 kDa Exposure time: 100.0 s
Recombinant Smad2 Antibody (Ab128194) - IF/ICC Immunofluorescent analysis of HeLa cells, using Recombinant Smad2 Antibody (Ab128194).