DNA-binding protein APRF|HIES|signal transducer and activator of transcription 3 (acute-phase responsefactor)|signal transducer and activator of transcription 3|STAT3|Acute-phase response factor|Acute Phase Response Factor antibody|Acute-phase response factor antibody|ADMIO antibody|APRF antibody|AW109958 antibody|DNA binding protein APRF antibody|FLJ20882 antibody|HIES antibody|MGC16063 antibody|Signal transducer and activator of transcription 3 (acute phase response factor) antibody|Signal transducer and activator of transcription 3 antibody|STAT 3 antibody|Stat3 antibody
规格或纯度
重组, ExactAb™, 敲低验证, 已验证, 见COA
生化机理
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
宿主种属
兔(Rabbit)
特异性
STAT3
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human STAT3 (AA 670-700)
阳性对照
WB: Mouse heart, HT-1080, HeLa, HEK293, A375, A431, NIH/3T3 and C6 lysates. IHC: Rat brain, Human astrocytoma, Human Hodgkin's lymphoma tissues. IF/ICC: HeLa cells.
Recombinant STAT3 Antibody (Ab129421) - Western Blot All lanes: Recombinant STAT3 Antibody (Ab129421) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 91 kDa Exposure time: 5.2 s
Recombinant STAT3 Antibody (Ab129421) - Western Blot All lanes use Recombinant STAT3 Antibody (Ab129421) at 1/1000 dilution for 1 hour at room temperature.
Recombinant STAT3 Antibody (Ab129421) - IF/ICC Immunofluorescent analysis of Hela cells treated with IFN alpha, using Recombinant STAT3 Antibody (Ab129421).
Recombinant STAT3 Antibody (Ab129421) - IHC Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using Recombinant STAT3 Antibody (Ab129421) at 1/100 dilution.
Recombinant STAT3 Antibody (Ab129421) - IHC Immunohistochemical analysis of paraffin-embedded Human astrocytoma, using Recombinant STAT3 Antibody (Ab129421) at 1/100 dilution.
Recombinant STAT3 Antibody (Ab129421) - IHC Immunohistochemical analysis of paraffin-embedded rat brain, using Recombinant STAT3 Antibody (Ab129421).
应用
应用名称
稀释比例
IHC
1/100 - 1/200
IF/ICC
1/50 - 1/200
WB
1/1000-1/2000
高级数据
关联靶点（人）
STAT3Tchem信号转导和转录激活因子 3(Signal transducer and activator of transcription 3)
(0 活性数据)
ZJ24F0708504
危险品化学品经营许可证（带存储）