Rabbit anti Human STAT5a Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 2.5 µg/mL
Protein Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
规格
产品名称
STAT5a Antibody
别名
信号转导和转录激活因子5a多克隆抗体 | STAT5a 抗体
英文别名
Mammary gland factor antibody | MGF antibody | Signal transducer and activator of transcription 5A antibody | STA5A_HUMAN antibody | STAT 5 antibody | STAT 5A antibody | STAT5 antibody | STAT5A antibody | MGF | signal transducer and activator of transcrip
规格或纯度
无载体, ExactAb™, 已验证, 高性能, 见COA
宿主种属
兔(Rabbit)
特异性
STAT5a
种属反应性
人(Human),小鼠(Mouse),猪(Pig)
免疫原
Recombinant STAT5a expressed in E.coli (AA 673-773)
阳性对照
WB: MOLT-4 and K562 cell lysates. IHC: Human lymph node tissue. IF/ICC: HeLa cells.
偶联
Unconjugated
克隆类型
多克隆抗体
Format
Whole IgG
亚型
Rabbit IgG
纯化方法
Antigen affinity purified and Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10 mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂
0.02% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
图片
STAT5a Antibody (Ab129464) - Western Blot All lanes: STAT5a Antibody (Ab129464) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time: 24.2 s
STAT5a Antibody (Ab129464) - IHC DAB staining on IHC Sample: Human Lymph node Tissue Primary Ab: 20 μg/mL STAT5a Antibody (Ab129464) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
STAT5a Antibody (Ab129464) - IF/ICC
IF analysis of STAT5a Antibody (Ab129464) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with STAT5a Antibody (Ab129464) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ24F1011794
危险品化学品经营许可证（带存储）