Rabbit anti Human TIGAR Antibody, Polyclonal, could be used for WB and so on. Application:
WB: 1/500-1/2000
IF/ICC: 1/200 Protein Function Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53.
TIGAR Antibody (Ab131228) - Western Blot All lanes: TIGAR Antibody (Ab131228) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 30 kDa Observed band size: 26 kDa Exposure time: 10.0 s
TIGAR Antibody (Ab131228) - IF/ICC IF analysis of TIGAR Antibody (Ab131228) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with TIGAR Antibody (Ab131228) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).