52 kDa ribonucleoprotein autoantigen Ro/SS-A antibody | 52 kDa Ro protein antibody | 52kD Ro/SSA autoantigen antibody | Autoantigen Ro/SSA, 52-KD antibody | E3 ubiquitin-protein ligase TRIM21 antibody | RING finger protein 81 antibody | RNF81 antibody | R
规格或纯度
重组, ExactAb™, 已验证, 见COA
生化机理
E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.
宿主种属
兔(Rabbit)
特异性
TRIM21/SS-A
种属反应性
人(Human)
免疫原
Recombinant protein of human TRIM21 (AA 1-242)
阳性对照
WB: K562, HeLa, Jurkat, HEK293 and MOLT-4 cell lysates.
Recombinant TRIM21/SS-A Antibody (Ab132468) - Western Blot All lanes: Recombinant TRIM21/SS-A Antibody (Ab132468) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 54 kDa Observed band size: 49 kDa Exposure time: 105.5 s
ZJ24F0303477
危险品化学品经营许可证（带存储）