Recombinant TSG101 Antibody (Ab132811) - Western Blot All lanes: Recombinant TSG101 Antibody (Ab132811) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa Observed band size: 46, 53 kDa The doublet are consistent with what have been described in the literature (PMID: 27882925, 31684046, 32932791, 11427703). Exposure time: 6.0 s
Recombinant TSG101 Antibody (Ab132811) - IHC Immunohistochemical analysis of paraffin-embedded Mouse kidney, using Recombinant TSG101 Antibody (Ab132811) at 1/1000 dilution.
Recombinant TSG101 Antibody (Ab132811) - IHC Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using Recombinant TSG101 Antibody (Ab132811) at 1/200 dilution.
Recombinant TSG101 Antibody (Ab132811) - IHC Immunohistochemical analysis of paraffin-embedded Human tongue cancer, using Recombinant TSG101 Antibody (Ab132811) at 1/200 dilution.
Recombinant TSG101 Antibody (Ab132811) - IHC Immunohistochemical analysis of paraffin-embedded Rat stomach, using Recombinant TSG101 Antibody (Ab132811) at 1/200 dilution.
Recombinant TSG101 Antibody (Ab132811) - IHC Immunohistochemical analysis of paraffin-embedded Rat liver, using Recombinant TSG101 Antibody (Ab132811) at 1/200 dilution.
Recombinant TSG101 Antibody (Ab132811) - IF/ICC IF analysis of Recombinant TSG101 Antibody (Ab132811) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant TSG101 Antibody (Ab132811) at 1/400 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).