Rabbit anti Human UBE2B Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/2000 Protein Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
规格
产品名称
Recombinant UBE2B Antibody
别名
泛素激活酶2B抗体 | 泛素激活酶2B抗体 | 重组UBE2B抗体
英文别名
E2 17kDa antibody | E2 protein antibody | hHR6B antibody | HR6B antibody | RAD6 homolog B antibody | RAD6B antibody | UBC2 antibody | UBE2B antibody | UBE2B_HUMAN antibody | Ubiquitin carrier protein B antibody | Ubiquitin conjugating enzyme E2 B antibody
规格或纯度
重组, ExactAb™, 已验证, 见COA
宿主种属
兔(Rabbit)
特异性
UBE2B
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthesized peptide derived from human UBE2B (AA 130-152)
阳性对照
WB: Jurkat, K562, Hep G2, NIH/3T3 and C6 cell lysates.
Recombinant UBE2B Antibody (Ab133222) - Western Blot All lanes: Recombinant UBE2B Antibody (Ab133222) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 20 kDa Exposure time: 62.3 s