Rabbit anti Human Ube2N / Ubc13 Antibody, Recombinant, could be used for WB and so on. Application · WB: 1/500-1/1000 Protein Function The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
规格
产品名称
Recombinant Ube2N/Ubc13 Antibody
别名
泛素蛋白连接酶2N抗体 | 重组Ube2N/Ubc13抗体
英文别名
Bendless-like ubiquitin-conjugating enzyme | E2 ubiquitin-conjugating enzyme N | EC 2.3.2.23 | Ubc13 | UbcH13 | Ubiquitin carrier protein N | Ubiquitin-protein ligase N | Bendless like ubiquitin conjugating enzyme antibody | Bendless-like ubiquitin-conjug
规格或纯度
ExactAb™, Validated, 重组, 0.7 mg/mL
宿主种属
兔(Rabbit)
特异性
UBE2N
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
A synthetic peptide derived from human Ube2N/Ubc13 (AA 1-50)
阳性对照
WB: Jurkat, Raji, HEK293, U2OS, NIH/3T3 and C6 cell lysates.
Recombinant Ube2N/Ubc13 Antibody (Ab133260) - Western Blot All lanes: Recombinant Ube2N/Ubc13 Antibody (Ab133260) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 17 kDa Observed band size: 16 kDa Exposure time: 63.9 s