Rabbit anti Human Xanthine Oxidase Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500 - 1/1000 Protein Function Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Post-translational Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Recombinant Xanthine Oxidase Antibody (Ab134849) - Western Blot All lanes: Recombinant Xanthine Oxidase Antibody (Ab134849) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 146 kDa Observed band size: 142 kDa Exposure time: 68.3 s
Recombinant Xanthine Oxidase Antibody (Ab134849) - Western Blot All lanes: Recombinant Xanthine Oxidase Antibody (Ab134849) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 146 kDa Observed band size: 142 kDa Exposure time: 10.0 s