ExactAb™,PBS Only,已验证,无叠氮钠,无载体,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Application：
WB: 1/1,000-1/20,000; Predicted molecular weight: 42 kDa.
Flow: 0.016-2.0μg/mL
ICC/IF: 0.5μg/mL
Protein Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
0.016-2.0μg/mL
0.5 μg/mL
1/1,000-1/20,000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0910105 |
分析证书 | Ab155822 |
| ZJ24F0404947 |
分析证书 | Ab155822 |
| ZJ24F0404948 |
分析证书 | Ab155822 |
| ZJ24F0404949 |
分析证书 | Ab155822 |
| ZJ24F0404950 |
分析证书 | Ab155822 |
| ZJ23F0700608 |
分析证书 | Ab155822 |
| ZJ23F0700609 |
分析证书 | Ab155822 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Carrier Free 级别指南 → 查看 Validated 级别指南 → 查看 ExactAb™ 级别指南 → 查看 Azide Free 级别指南 → 查看 High Performance 级别指南 → 查看 PBS Only 级别指南 →
危险品化学品经营许可证（带存储）