| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab155822-10μl (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！
|
10μl |
现货  |
| |
| Ab155822-50μl |
50μL |
现货 |
| |
| Ab155822-100μl |
100μL |
现货 |
| |
| Ab155822-1ml |
1ml |
现货 |
|
| 产品名称 | beta Actin Mouse mAb |
|---|---|
| 别名 | β actin antibody | β-actin antibody | beta Actin 抗体 | Actin 抗体 | β肌动蛋白小鼠单克隆抗体 | β肌动蛋白小鼠单抗 |
| 英文别名 | A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | beta actin |
| 应用 | WB,Flow,ICC/IF,ELISA |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | 兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken) |
| 免疫原 | Synthetic peptide in beta Actin |
| 阳性对照 | WB: A549, MCF-7, HeLa, Hep G2, Raji, Ramos, MOLT-4, Jurkat, LNCap, A431, HEK293, NIH/3T3, K562, HT-29, THP-1 lysates. ICC/IF: HeLa cells. Flow: HeLa cells. |
| 偶联 | Unconjugated |
| 抗体类型 | Primary antibody |
|---|---|
| 克隆类型 | 单克隆抗体 |
| 克隆号 | FG01/2D10 |
| Format | Whole IgG |
| 亚型 | IgG1 |
| 轻链亚型 | kappa |
| 纯化方法 | Protein A purified |
| 来源 | Hybridoma supernatant |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 防腐剂 | No |
| 浓度 | 见COA |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 +4°C 下短期储存（1-2 周）。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
| 应用名称 | 稀释比例 |
|---|---|
| Flow Cytometry |
0.016-2.0μg/mL
|
| IF/ICC |
0.5 μg/mL
|
| WB |
1/1,000-1/20,000
|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ24F0910105 |
分析证书 | 24-09-04 | Ab155822 |
| ZJ24F0404950 |
分析证书 | 24-04-26 | Ab155822 |
| ZJ24F0404949 |
分析证书 | 24-04-26 | Ab155822 |
| ZJ24F0404948 |
分析证书 | 24-04-26 | Ab155822 |
| ZJ24F0404947 |
分析证书 | 24-04-26 | Ab155822 |
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