Induces caspases and apoptosis.
Counters the protective effect of BCL2 (By similarity).
BH3-interacting domain death agonist p15, Induces caspase activation and apoptosis.
Allows the release of cytochrome c.
Isoform 1, Induces ICE-like proteases and apoptosis.
Isoform 2, Induces ICE-like proteases and apoptosis.
Isoform 3, Does not induce apoptosis.
Isoform 4, Induces ICE-like proteases and apoptosis.
宿主种属
小鼠(Mouse)
特异性
Human BID
种属反应性
人(Human)
免疫原
Recombinant protein corresponding to Human BID
阳性对照
WB: Jurkat, HepG2, MCF-7, A549 and MOLT-4 whole cell lysates IF/ICC: HeLa cells
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
BID Mouse mAb (Ab214361) - Western Blot All lanes: BID Mouse Antibody (Ab214361) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 60.0 s
BID Mouse mAb (Ab214361) - ICC/IF IF analysis of BID (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with BID Mouse mAb (Ab214361) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.