计算溶液所需的质量、体积或浓度。
Recombinant beta Dystroglycan Antibody (Ab336566) - Western Blot
All lanes: Recombinant beta Dystroglycan Antibody (Ab336566) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 17, 26, 40 kDa
Exposure time: 120.0 s
A novel nuclear localization of β-dystroglycan has been identified, and its phosphorylated form may participate in nuclear functions. In the non-phosphorylated state, only the full-length 43 kDa β-dystroglycan enters the nucleus, while the 26 kDa fragment remains in the cytoplasm. Upon tyrosine phosphorylation, β-dystroglycan not only shows increased nuclear accumulation but also exhibits multiple molecular weight species (likely due to degradation or post-translational modifications), including newly identified 17 kDa and 50 kDa fragments in addition to the previously reported forms. These findings suggest that phosphorylation regulates the subcellular localization and processing of dystroglycan.(PMID: 24077328; 14678802; 18458097; 17255331)
危险品化学品经营许可证(带存储)