蛋白酶抑制剂（Protease Inhibitor）可与蛋白酶分子活性中心的特定基团结合，在不使蛋白变性的前提下降低或消除酶活力，从而有效抑制蛋白降解、维持蛋白间天然相互作用。常见蛋白酶抑制剂包括 EDTA、E-64、NaVO₃、Bestatin、Leupeptin、Pepstatin A、Aprotinin 等。
亮抑酶肽溶液（Leupeptin, 1 mg/mL）以亮抑酶肽（Leupeptin）为主要成分，不含 EDTA，为浓缩液；其工作浓度为1～10 μg/mL，常用浓度3～5 μg/mL，适用于哺乳动物组织、细胞的蛋白质提取，可高效获得目的蛋白，提取产物可用于 Western Blot、免疫共沉淀等后续实验。 注：本试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）
开盖前低速离心，使黏附于管壁的液体沉降至管底。
根据裂解液用量加入本品，稀释至目标工作浓度（1～10 μg/mL，推荐 3～5 μg/mL）后使用。
注意事项
本品具有轻微刺激性，操作需谨慎，同时避免试剂反复冻融。
为保障安全与健康，实验时请穿戴实验服及一次性手套。
试剂开封后建议尽快使用，以免影响实验效果。
Protease inhibitors can bind to specific groups in the active centers of protease molecules, reducing or eliminating enzyme activity without denaturing the proteins. This effectively inhibits protein degradation and maintains native protein-protein interactions. Common protease inhibitors include EDTA, E-64, NaVO₃, Bestatin, Leupeptin, Pepstatin A, Aprotinin, etc.
Product Description
The Leupeptin Solution (1 mg/mL) is primarily composed of leupeptin. It is EDTA-free and provided as a concentrated solution. Its working concentration ranges from 1 to 10 μg/mL, with a commonly used concentration of 3 to 5 μg/mL. It is suitable for protein extraction from mammalian tissues and cells, facilitating efficient obtainment of target proteins. The extracted products can be used in subsequent experiments such as Western Blot, Co-Immunoprecipitation (Co-IP), etc.
This reagent is for research use only. Not for use in diagnostic procedures or other purposes.
Procedure (For Reference Only)
Briefly centrifuge the tube before opening to spin down any liquid adhering to the walls or cap.
Add this product to the required volume of Lysis Buffer and dilute to the desired target working concentration (1–10 μg/mL, 3–5 μg/mL recommended) before use
Notes
This product is mildly irritating. Handle with care and avoid repeated freezing and thawing.
For your safety and health, please wear laboratory appropriate attire and disposable gloves while handling reagents.
Use the reagent as soon as possible after opening to ensure optimal performance.
ZJ26F0838221
危险品化学品经营许可证（带存储）