计算溶液所需的质量、体积或浓度。
BioReagent,植物样品抽提用 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
叶绿体是光合作用的细胞器,在光合作用研究中,常需要用提取的叶绿体展开下游研究工作,叶绿体中所含的色素主要有两大类,叶绿素 (包括叶绿素a和叶绿素b)和类胡萝卜素 (包括胡萝卜素和叶黄素),它们与类囊体膜上的蛋白质结合,成为色素蛋白复合体,其中叶绿素又称叶绿体色素。
叶绿素提取试剂的抽提原理是叶绿素不溶解于水,而溶于有机溶剂,以有机溶剂粗提叶绿素,主要用于植物组织中叶绿素的提取,及叶绿素含量的检测。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
产品组成:
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自备材料:
1、电子天平、研钵或匀浆器、离心管、滤纸或纱布
2、待检样品、去离子水
操作步骤 (仅供参考):
1、取菠菜或其他植物新鲜叶片,洗净,擦干,去中脉,称取剪碎的新鲜样品0.1 g,置于研钵或匀浆器,按照0.1 g (剪碎的样品):50mg (试剂B): 1 ml (试剂A)的比例混合,研磨或匀浆成液态。
2、将研磨液或匀浆液转移至10 ml离心管,用少量叶绿素提取试剂 (试剂A)冲洗研钵或匀浆器数次,最后连残渣一同倒入10 ml离心管,补加叶绿素提取试剂至10 ml,混匀,避光放置5 min-2 h。注意:如果大量提取叶绿素,应以每0.1 g组织补加叶绿素提取试剂至10 ml的比例扩大提取体积。
3、观察底部组织残渣接近于白色,即为提取完全,如果仍有较多组织颜色,应再加入叶绿素提取试剂继续避光放置。
4、滤纸或三层纱布过滤,留取滤液,即为叶绿素粗提液。
注意事项:
1、为了避免叶绿素见光分解,操作时应尽量避光,研磨或匀浆时应尽量缩短时间。
2、测定吸光度值大于1时,可适当稀释后再行测定。
3、叶绿素粗提液不能出现浑浊现象,否则应重新过滤。
4、试剂开封后请尽快使用,以防影响后续实验效果。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Chloroplasts are the organelles responsible for photosynthesis. In photosynthesis research, it is often necessary to use isolated chloroplasts for downstream studies. The pigments contained in chloroplasts mainly fall into two categories: chlorophylls (including chlorophyll a and chlorophyll b) and carotenoids (including carotenes and xanthophylls). They bind to proteins on the thylakoid membrane to form pigment-protein complexes. Among them, chlorophyll is also known as chloroplast pigment.
The extraction principle of Chlorophyll Extraction Reagent is that chlorophyll is insoluble in water but soluble in organic solvents. This reagent is used for the crude extraction of chlorophyll with organic solvents, mainly for the extraction of chlorophyll from plant tissues and the determination of chlorophyll content.This reagent is for research use only and is not suitable for clinical diagnosis or other applications.
Components
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Materials Provided by User
1. Electronic balance, mortar or homogenizer, centrifuge tube, filter paper or gauze
2. Test sample, deionized water
Protocol (For Reference Only)
1. Take fresh leaves of spinach or other plants, wash and blot dry, remove the midribs. Weigh 0.1 g of cut fresh sample into a mortar or homogenizer. Mix at a ratio of 0.1 g (cut sample): 50 mg (Reagent B): 1 mL (Reagent A), then grind or homogenize into a liquid.
2. Transfer the homogenate to a 10 mL centrifuge tube. Rinse the mortar or homogenizer several times with a small amount of Chlorophyll Extraction Reagent (Reagent A), and pour all rinsing solution and residue into the 10 mL centrifuge tube. Add Chlorophyll Extraction Reagent to a final volume of 10 mL, mix well, and keep in the dark for 5 min-2 h.
Note: For large‑scale chlorophyll extraction, scale up the volume according to the ratio of adding Chlorophyll Extraction Reagent to 10 mL per 0.1 g of tissue.
3. Extraction is complete when the tissue residue at the bottom is nearly white. If the tissue is still strongly colored, add more Chlorophyll Extraction Reagent and keep in the dark.
4. Filter through filter paper or three layers of gauze. The filtrate obtained is the crude chlorophyll extract.
Precaution
1. To avoid photodegradation of chlorophyll, perform all procedures in the dark as much as possible, and minimize the grinding or homogenization time.
2. If the absorbance value is greater than 1, dilute the sample appropriately before measurement.
3. The crude chlorophyll extract must not be turbid; otherwise, filter again.
4. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1518212 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for plant cell and tissue extracts 级别指南 →