环保浸蜡脱蜡透明液又称组织透明剂、脱蜡透明剂、脱蜡透明液等，是一种专门为组织病理学和细胞学样本处理开发的无色无味、挥发速率低、具有生物降解性的有机溶剂，不含芳香族化合物，是一种较理想的环保型透明剂，可取代组织制片过程中的二甲苯。
石蜡是一种有机化合物，根据相似相溶原理，环保透明剂可对组织样本进行透明，有利于浸蜡，也可溶解组织中的石蜡从而起到脱蜡作用，该试剂在使用中对仪器无腐蚀性，是一种使用效果较佳的绿色环保型产品，可大大改善实验工作环境，保障工作人员的身心健康主要用于浸蜡组织样本的处理及脱蜡、透明以及细胞学制片的透明。
操作步骤(仅供参考)：
组织切片常规HE染色：
1、浸蜡脱蜡透明液脱蜡 (l) 5~15分钟
2、浸蜡脱蜡透明液脱蜡 (II) 5~15分钟
3、无水乙醇 2~3分钟
4、95%乙醇 1~2分钟
5、80%乙醇 1~2分钟
6、水洗 1~2分钟
7、苏木素-伊红染色（根据具体实验进行操作）
8、脱水（根据具体实验进行操作）
9、浸蜡脱蜡透明液透明 (l) 2~5分钟
10、浸蜡脱蜡透明液透明 (II) 2~5分钟
11、封片胶封固
组织处理 （组织脱水机）
1、10%中性福尔马林固定 (l) 2小时
2、10%中性福尔马林固定 (II) 2小时
3、80%乙醇 45分钟
4、95%乙醇 (I) 45分钟
5、95%乙醇 (II) 45分钟
6、无水乙醇 (I) 45分钟
7、无水乙醇 (II) 45分钟
8、浸蜡脱蜡透明液透明 (I) 1小时
9、浸蜡脱蜡透明液透明 (II) 1小时
10、65℃浸蜡 (I) 1小时
11、65℃浸蜡 (II) 1小时
12、65℃浸蜡 (III) 2~3小时
样本要求:无论用于组织浸蜡还是染色后的透明，均应充分脱水后才可使用。
注意事项:
1、初次使用浸蜡脱蜡透明液时，请同时更换浸蜡石蜡。
2、浸蜡脱蜡透明液应视使用情况进行更新，以平均每天300片组织蜡块的量为例，应7~10个工作日更换1次。
3、浸蜡脱蜡透明液同样适用于细胞学制片巴氏染色程序的透明，也适用于冰冻快速制片程序的透明(使用时应酌情缩短透明时间)。
4、浸蜡脱蜡透明液浸蜡组织处理及脱蜡、透明后的组织样本制片其染色效果均不会影响，也不会影响组织切片(细胞涂片)的保存。
5、脱蜡时，一般建议在60~70℃温箱中处理。
6、试剂开封后请尽快使用，以防影响后续实验效果。
7、该试剂仅用于科研领域，不适用于临床诊断或其他用途。
Environment-friendly Wax-Soaking and Dewaxing Clearing Solution, also known as tissue clearing agent, dewaxing clearing agent, dewaxing clearing solution, etc., it is a colorless, odorless, low-volatility, and biodegradable organic solvent specially developed for the processing of histopathological and cytological specimens. Free of aromatic compounds, it is an ideal environmentally friendly clearing agent that can replace xylene in tissue preparation.
Paraffin is an organic compound. Based on the principle of like dissolves like, the environment-friendly clearing solution can clear tissue specimens to facilitate wax infiltration, and can also dissolve paraffin in tissues to achieve dewaxing. The reagent is non-corrosive to instruments during use. As a high-performance green and environment-friendly product, it can greatly improve the experimental working environment and protect the physical and mental health of laboratory personnel. It is mainly used for the processing of wax-soaked tissue specimens, dewaxing and clearing, as well as clearing in cytological preparation.
Protocol (for reference only):
Routine HE Staining of Tissue Sections:
1. Dewax with Environment-friendly Wax-Soaking and Dewaxing Clearing Solution (I) for 5–15 minutes
2. Dewax with Environment-friendly Wax-Soaking and Dewaxing Clearing Solution (II) for 5–15 minutes
3. Absolute ethanol for 2–3 minutes
4. 95% ethanol for 1–2 minutes
5. 80% ethanol for 1–2 minutes
6. Water rinse for 1–2 minutes
7. Hematoxylin and eosin (HE) staining (operate according to specific experiments)
8. Dehydration (operate according to specific experiments)
9. Clearing with Environment-friendly Wax-Soaking and Dewaxing Clearing Solution (I) for 2–5 minutes
10. Clearing with Environment-friendly Wax-Soaking and Dewaxing Clearing Solution (II) for 2–5 minutes
11. Mounting with mounting medium
Tissue Processing (Tissue Processor):
1. Fixation with 10% neutral buffered formalin (I) for 2 hours
2. Fixation with 10% neutral buffered formalin (II) for 2 hours
3. 80% ethanol for 45 minutes
4. 95% ethanol (I) for 45 minutes
5. 95% ethanol (II) for 45 minutes
6. Absolute ethanol (I) for 45 minutes
7. Absolute ethanol (II) for 45 minutes
8. Clearing with Environment-friendly Wax-Soaking and Dewaxing Clearing Solution (I) for 1 hour
9. Clearing with Environment-friendly Wax-Soaking and Dewaxing Clearing Solution (II) for 1 hour
10. Wax infiltration at 65℃ (I) for 1 hour
11. Wax infiltration at 65℃ (II) for 1 hour
12. Wax infiltration at 65℃ (III) for 2–3 hours
Sample Requirements: Tissues must be adequately dehydrated before use, whether for tissue wax infiltration or clearing after staining.
ZJ26F0737197
危险品化学品经营许可证（带存储）