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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
干 扰 素 基 因 刺 激 蛋 白 (STING) 又 称 跨 膜 蛋 白 173 ( TMEM173 ) 和 MPYS/MITA/ERIS,是一种由 STING1 基因编码的蛋白质。它在先天免疫中 发挥着重要作用。当细胞感染细胞内病原体,如病毒、霉菌和细胞内寄生虫 时,它会诱导 I 型干扰素的产生。由它介导的 I 型干扰素通过与分泌它的同一 细胞(自分泌信号)和附近的细胞(旁分泌信号)结合,保护受感染的细胞 和附近的细胞免受局部感染。因此它在控制诺如病毒感染等方面发挥了重要 作用。它通过不同的分子机制,在 I 型干扰素信号传导中既是直接的细胞膜 DNA 传感器(CDS),又是适应蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人干扰素基因刺激蛋白(STING)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人干扰素基因刺激蛋白(STING)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human STING. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human STING present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.