计算溶液所需的质量、体积或浓度。
改良Weigert弹力纤维染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
弹力纤维(ElasticFiber)主要分布于人体的动脉壁、肺泡壁、皮肤，新鲜时呈黄色，折光性强，常用的弹力纤维染色法有Gomori醛品红法、间苯二酚碱性品红法、地衣红法、维多利亚蓝法、铁碘苏木素法等。改良Weigert弹力纤维染色液主要由间苯二酚品红染色液和改良VG染色液等组成，间苯二酚碱性品红染色液主要用于弹力纤维染色，又称Weigert树脂酚复红染色液，VanGieson染色法主要用于胶原纤维的染色，其染色原理与阴离子染料分子的大小和组织的渗透有关；PA分子量小，丽春红和复红次之，淡绿分子量最大；VG染色后，肌纤维呈黄色，胶原纤维呈红色。
改良Weigert弹力纤维染色液染色原理在于间苯二酚品红与铁离子形成Lake复合物，其游离的氨基与弹力纤维内的氢键牢固结合呈黑色，较常规Weigert间苯二酚品红弹力纤维染色多了氧化和漂白过程，适用于显示各种弹力纤维，比传统的Weigert间苯二酚品红法更清晰、不易脱色、保存时间长。本产品可显示皮肤组织中弹力纤维的变化，如弹力纤维痣、皮肤环状肉芽肿、硬度病等；显示与判定心内膜及动脉的病变，观察某些病变中是击伴有弹力纤维的增生或破坏；还可鉴别肿瘤组织成分如弹力纤维瘤，经弹力纤维染色后，可清晰见到瘤体内弹力纤维球。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| I1518582 | Component | 5×50mL | Storage |
| I1518582A | Weigert 氧化剂 A | 25mL | 2-8℃. Store in the dark. |
| I1518582B | Weigert 氧化剂 B | 25mL | RT |
| I1518582C | Weigert 漂白剂 | 50mL | RT |
| I1518582D | Weigert 间苯二酚品红染色液 | 50mL | 2-8℃. Store in the dark. |
| I1518582E | 酸性分化液 | 50mL | RT |
| I1518582F | 丽春红 S溶液 | 5mL | RT |
| I1518582G | PA溶液 | 45mL | RT |
临用前，取A、B等量混合即为Weigert 氧化剂，不宜提前配制。
临用前，按F:G=1:9混合即为VG染色液，不宜提前配制。
自备材料：
1、10%福尔马林固定液、系列乙醇、、二甲苯或环保脱蜡透明液、中性树胶
2、染色缸、恒温箱
操作步骤(仅供参考)：
1、组织固定于10%的福尔马林中，常规脱水包埋。
2、切片厚4μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、用配制好的Weigert氧化剂氧化5min，稍水洗。
4、用Weigert漂白剂漂白1~2min，流水冲洗2min，95%的乙醇稍洗。
5、切片入装有Weigert间苯二酚品红染色液的染色缸(加盖)中，室温下染色1～3h或56°C下0.5~1h。
6、用酸性分化液分化至无染液脱下，约2～3s，流水冲洗10min。
7、VG染色液复染30s，用水急速洗一下，立刻用95%乙醇快速分化和脱水。
8、无水乙醇脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
弹力纤维：蓝色至紫黑色
胶原纤维：红色
肌纤维和红细胞：黄色
注意事项：
1、如仅显示成熟的弹性纤维，可省略上述氧化和漂白步骤。
2、酸性分化液主要使切片背景清晰，分化稍长一些对染色无损害。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Elastic fibers are mainly distributed in the arterial wall, alveolar wall, and skin of the human body. They appear yellow when fresh and have strong refraction. Common elastic fiber staining methods include Gomori Aldehyde Fuchsin, Resorcinol-Fuchsin, Orcein, Victoria Blue, and Iron-Iodine Hematoxylin. Modified Weigert Elastic Fiber Staining Solution is mainly composed of Resorcinol-Fuchsin Staining Solution and modified Van Gieson (VG) Staining Solution. Resorcinol-Fuchsin Staining Solution is mainly used for elastic fiber staining and is also known as Weigert Resorcinol Fuchsin Staining Solution. Van Gieson staining is mainly used for collagen fiber staining. Its staining principle is related to the molecular size of anionic dyes and tissue permeability: Picric acid (PA) has the smallest molecular weight, followed by Ponceau and Fuchsin, while Light Green has the largest. After VG staining, muscle fibers appear yellow and collagen fibers appear red.
Modified Weigert Elastic Fiber Staining Solution is that resorcinol-fuchsin forms a lake complex with iron ions, and its free amino groups bind firmly to hydrogen bonds in elastic fibers, appearing black. Compared with the conventional Weigert resorcinol-fuchsin method, this modified method adds oxidation and bleaching steps. It is suitable for demonstrating various elastic fibers, with clearer staining, less fading, and longer storage time. This product can demonstrate changes of elastic fibers in skin tissue, such as elastic nevus, granuloma annulare, and scleroderma; demonstrate and evaluate endocardial and arterial lesions, and observe whether some lesions are accompanied by hyperplasia or destruction of elastic fibers. It can also identify tumor components such as elastofibroma, in which elastic fiber globules can be clearly seen after elastic fiber staining. This reagent is for research use only and not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| I1518582 | Component | 5×50mL | Storage |
| I1518582A | Weigert Oxidant A | 25mL | 2-8℃. Store in the dark. |
| I1518582B | Weigert Oxidant B | 25mL | RT |
| I1518582C | Weigert Bleaching Agent | 50mL | RT |
| I1518582D | Weigert Resorcinol-Fuchsin Staining Solution | 50mL | 2-8℃. Store in the dark. |
| I1518582E | Acid Differentiation Solution | 50mL | RT |
| I1518582F | Ponceau S Solution | 5mL | RT |
| I1518582G | PA Solution | 45mL | RT |
Immediately before use, mix equal volumes of Solution A and Solution B to prepare Weigert Oxidant. Do not prepare in advance.
Immediately before use, mix Solution F and Solution G at a ratio of 1:9 to prepare VG Staining Solution. Do not prepare in advance.
Materials Required (User-supplied):
1. 10% Formalin Fixative, graded ethanol, xylene or eco-friendly dewaxing clearing agent, neutral balsam
2. Staining jars, incubator
Protocol (For Reference Only):
1. Fix tissues in 10% formalin, followed by routine dehydration and embedding.
2. Cut 4‑μm sections, dewax with xylene or eco‑friendly dewaxing clearing agent, and hydrate to water.
3. Oxidize with freshly prepared Weigert Oxidant for 5 min, then rinse briefly with water.
4. Bleach with Weigert Bleaching Agent for 1–2 min, wash under running water for 2 min, then rinse briefly with 95% ethanol.
5. Place sections in a staining jar (capped) containing Weigert Resorcinol‑Fuchsin Staining Solution. Stain for 1–3 h at room temperature or 0.5–1 h at 56 °C.
6. Differentiate in Acid Differentiation Solution until no more stain elutes (about 2–3 s), then wash under running water for 10 min.
7. Counterstain with VG Staining Solution for 30 s, rinse rapidly with water, then immediately differentiate and dehydrate quickly with 95% ethanol.
8. Dehydrate in absolute ethanol, clear with xylene or dewaxing clearing agent, and mount with neutral balsam.
Staining Results:
Elastic fibers: Blue to bluish‑black
Collagen fibers: Red
Muscle fibers and erythrocytes: Yellow
Precautions:
1. The oxidation and bleaching steps may be omitted if only mature elastic fibers are to be demonstrated.
2. Acid Differentiation Solution mainly clarifies the background; slightly prolonged differentiation does not harm the staining.
3. Please use the reagents as soon as possible after opening to avoid affecting experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
弹力纤维间苯二酚碱性品红染色
英文别名
Modified Weigert Elastane Staining Solution set
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Modified Weigert Elastic Fiber Staining Solution
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0535343 |
分析证书 | I1518582 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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