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Russell改良Movat五色套染染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
结缔组织狭义上是指其含有的三种纤维：胶原纤维、网状纤维、弹力纤维，结缔组织染色方法亦有很多种，如Masson三色染色法、VanGieson染色法、Gomori氨银法、Mallory磷钨酸苏木素染色，然而以上染色方法只是侧重于某一两种组织的染色。
Russell改良Movat五色套染以其染色丰富、鲜艳而大受欢迎，该染色法主要用于显示动脉粥样硬化斑块；Weigert苏木素用于染细胞核，藏红品红用于染细胞质，藏红花染胶原组织，阿尔辛蓝染基质(蛋白聚糖)；由于该试剂盒操作过程复杂，其染色效果跟操作者经验和数量程度有很大关系，所以同时染出十分满意的结果并不容易。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| R1518553 | Component | 9×50mL | Storage |
| R1518553A | 海波溶液 | 50mL | RT |
| R1518553B | 阿利新蓝染色液 | 50mL | 2-8℃. Store in the dark. |
| R1518553C | 碱性乙醇溶液 | 50mL | RT |
| R1518553D | 韦格氏 A 液 | 30mL | RT |
| R1518553E | 韦格氏 B 液 | 20mL | RT |
| R1518553F | 韦格氏 C 液 | 10mL | RT. Store in the dark. |
| R1518553G | 藏红品红 A | 40mL | RT |
| R1518553H | 藏红品红 B | 10mL | RT |
| R1518553I | 磷钨酸溶液 | 50mL | RT. Store in the dark. |
| R1518553J | 弱酸分化液 | 50mL | RT |
| R1518553K | 醇藏红花染色液 | 50mL | RT |
| R1518553L | Bouin 固定液 | 250mL | RT. Store in the dark. |
临用时，按D:E:F=3:2:1混合即为Weigert 苏木素，不可预先配制。
临用时，按G:H=4:1混合即为藏红品红染色液，不可预先配制。
自备材料：
1、系列乙醇、蒸馏水、二甲苯或环保脱蜡透明液、中性树胶
2、微波炉
操作步骤(仅供参考)：
1、石蜡切片常规，二甲苯或脱蜡透明液脱蜡，系列乙醇水化。
2、取适量Bouin固定液放入微波炉中，中度加热30～60s，立即放入切片处理10min。
3、流水冲洗10min。
4、切片入海波溶液处理5min，蒸馏水冲洗2～3次。
5、切片入阿利新蓝染色液中染色20min，流水冲洗2～5min。
6、用微波炉强火45～60°C预热碱性乙醇溶液后，将切片入碱性乙醇溶液中处理10min。
7、流水冲洗2～5min。
8、切片入预先配制好的Weigert苏木素染色液中，避光染色60min。
9、流水稍冲洗，蒸馏水冲洗2～3次，每次3～5min。
10、切片入预先配制好的试剂藏红品红染色液中，避光染色1min。
11、蒸馏水冲洗2～3次,每次3～5min。
12、将切片入磷钨酸溶液中处理5min，直接转入弱酸分化液中处理5min。
13、蒸馏水冲洗2～3次，每次3～5min。
14、脱水：95%乙醇1min，100%乙醇2次，每次1min。
15、将切片入藏红花染色液中，染色5min。
16、脱水：无水乙醇2次,每次1min，二甲苯或脱蜡透明液透明，覆盖玻片。
染色结果：
细胞核和弹力纤维：黑色
胶原蛋白和网状纤维：黄色
蛋白聚糖：蓝绿色
类纤维素、纤维素深：红色
心肌平滑肌：红色
泡沫细胞：紫色
注意事项：
1、由于染色力以及组织切片等原因，染色后未必显示出全部五种颜色。注意做防脱片处理。
2、切片厚度一般要求5μm左右，弹力纤维分化通常在2～3min内完成。
3、流水冲洗以除去Weigert苏木素染色液非常重要，若冲洗失败会抑制后续的染色步骤。
4、这种染色法可显示新型隐球菌，将其染成亮蓝色。
5、试剂开封后请尽快使用，以防影响后续实验效果。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
In a narrow sense, connective tissue refers to the three types of fibers it contains: collagen fibers, reticular fibers, and elastic fibers. There are many staining methods for connective tissue, such as Masson trichrome staining, Van Gieson staining, Gomori ammoniacal silver method, and Mallory phosphotungstic acid hematoxylin staining. However, the above methods only focus on the staining of one or two kinds of tissues.
Russell Modified Movat Pentachrome Staining Solution is widely used for its rich and bright staining. This method is mainly used to demonstrate atherosclerotic plaques. Weigert hematoxylin is used to stain cell nuclei, safranine fuchsin to stain cytoplasm, crocein scarlet to stain collagen tissue, and alcian blue to stain the matrix (proteoglycans). Due to the complicated operation procedure of this kit, the staining effect is highly related to the experience and proficiency of the operator, so it is not easy to obtain consistently satisfactory results. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| R1518553 | Component | 9×50mL | Storage |
| R1518553A | Hypo Solution | 50mL | RT |
| R1518553B | Alcian Blue Staining Solution | 50mL | 2-8℃. Store in the dark. |
| R1518553C | Alkalic Alcohol Solution | 50mL | RT |
| R1518553D | Weigert A | 30mL | RT |
| R1518553E | Weigert B | 20mL | RT |
| R1518553F | Weigert C | 10mL | RT. Store in the dark. |
| R1518553G | Safranin Fuchsin A | 40mL | RT |
| R1518553H | Safranin Fuchsin B | 10mL | RT |
| R1518553I | Phosphotungstic Acid Solution | 50mL | RT. Store in the dark. |
| R1518553J | Weak Acid Differentiation Solution | 50mL | RT |
| R1518553K | Alcoholic Crocein Scarlet Staining Solution | 50mL | RT |
| R1518553L | Bouin Fixative | 250mL | RT. Store in the dark. |
Immediately before use, mix D:E:F at a ratio of 3:2:1 to obtain Weigert hematoxylin. Do not prepare in advance.
Immediately before use, mix G:H at a ratio of 4:1 to obtain safranin fuchsin staining solution. Do not prepare in advance.
Materials Required:
1. Graded ethanol, distilled water, xylene or eco-friendly dewaxing and clearing agent, neutral balsam
2. Microwave oven
Protocol (For Reference Only):
1. Deparaffinize paraffin sections routinely with xylene or dewaxing and clearing agent, and rehydrate with graded ethanol.
2. Place an appropriate amount of Bouin fixative in a microwave oven, heat moderately for 30–60 seconds, then immediately immerse the sections for 10 minutes.
3. Rinse in running water for 10 minutes.
4. Treat sections in Hypo Solution for 5 minutes, then rinse 2–3 times with distilled water.
5. Stain sections in Alcian Blue Staining Solution for 20 minutes, then rinse in running water for 2–5 minutes.
6. Preheat Alkalic Alcohol Solution in a microwave oven at high power to 45–60 °C, then immerse sections for 10 minutes.
7. Rinse in running water for 2–5 minutes.
8. Stain sections in pre-mixed Weigert Hematoxylin Staining Solution for 60 minutes, protected from light.
9. Rinse briefly in running water, then wash 2–3 times with distilled water, 3–5 minutes each time.
10. Stain sections in pre-mixed Safranin Fuchsin Staining Solution for 1 minute, protected from light.
11. Rinse 2–3 times with distilled water, 3–5 minutes each time.
12. Treat sections in Phosphotungstic Acid Solution for 5 minutes, then transfer directly into Weak Acid Differentiation Solution for 5 minutes.
13. Rinse 2–3 times with distilled water, 3–5 minutes each time.
14. Dehydrate: 95% ethanol for 1 minute, 100% ethanol twice for 1 minute each.
15. Stain sections in Crocein Scarlet Staining Solution for 5 minutes.
16. Dehydrate: absolute ethanol twice for 1 minute each, clear in xylene or dewaxing and clearing agent, and coverslip.
Staining Results:
Nuclei and elastic fibers: Black
Collagen and reticular fibers: Yellow
Proteoglycans: bluish Green
Fibrinoid, deep cellulose: Deep red
Cardiac muscle and smooth muscle: Red
Foam cells: Purple
Precautions:
1. Due to staining intensity, tissue section quality and other factors, not all five colors may be displayed after staining. Anti-slip section treatment is recommended.
2. Section thickness is generally about 5 μm; elastic fiber differentiation is usually completed within 2–3 minutes.
3. Thorough rinsing in running water to remove Weigert Hematoxylin is critical; inadequate rinsing will inhibit subsequent staining steps.
4. This staining method can demonstrate Cryptococcus neoformans, which is stained bright blue.
5. Use reagents promptly after opening to avoid affecting experimental results.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
Russell改良Movat染色液
英文别名
Russell modified Movat color colored dye
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Russell Modified Movat Pentachrome Staining Solution
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0535352 |
分析证书 | R1518553 |
技术文档和文章
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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