计算溶液所需的质量、体积或浓度。
肌纤维染色液 (Puchtler鞣酸偶氮荧光桃红法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
肌纤维(Musclefiber)属于肌组织成分，由肌细胞组成，根据形态和功能特点，肌纤维可以分为平滑肌(又称横纹肌)、骨骼肌、心肌；肌纤维染色的方法有很多种，如丽春红法、苯胺蓝法、钨磷钨酸苏木素法等，鞣酸-偶氮荧光桃红法是利用两种酸性染料先后作用而完成鉴别染色。
肌纤维染色液 (Puchtler鞣酸偶氮荧光桃红法) 主要由苏木素染色液、鞣酸分化液、磷钼酸分化液、偶氮荧光桃红染色液等组成，其染色原理在于鞣酸容易进入渗透性强的胶原纤维，胶原纤维呈黄色；偶氮荧光桃红容易进入渗透性较低的肌纤维，肌纤维呈红色，该染色液用于区分肌纤维和胶原纤维，对比清晰，不宜褪色，同时可以显示肌上皮细胞，可用于乳腺和皮肤等肌上皮细胞瘤的诊断。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| M1518566 | Component | 5×50mL | Storage |
| M1518566A | 苏木素染色液 | 50mL | RT |
| M1518566B | 鞣酸分化液 | 50mL | RT |
| M1518566C | 磷钼酸分化液 | 50mL | RT . Store in the dark. |
| M1518566D | 偶氮荧光桃红染色液 | 50mL | 2-8℃. Store in the dark. |
| M1518566E | 酸性酒精分化液 | 50mL | RT |
自备材料：
1、Bouin固定液、Carnoy固定液、中性福尔马林等
2、蒸馏水、系列乙醇、、二甲苯或环保脱蜡透明液、中性树胶
操作步骤(仅供参考)：
1、常规组织固定于Bouin固定液(Carnoy固定液或中性福尔马林亦可)2～3h。
2、直接转入95%的乙醇中，常规脱水包埋。
3、石蜡切片厚4μm，常规二甲苯或脱蜡透明液脱蜡至水。
4、入苏木素染色液浸染8～10min，流水冲洗10min。
5、鞣酸分化液处理10min，流水冲洗1min。
6、磷钼酸分化液处理10min。
7、入偶氮荧光桃红染色液浸染10～15min。
8、酸性酒精分化液分化3～5s。
9、无水乙醇反复脱水多次，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
肌纤维、肌上皮细胞：玫瑰红
胶原纤维：黄色
细胞核：蓝色
注意事项：
1、尽量采用Bouin固定液固定组织，其他固定液有可能使染色较淡。
2、酸性酒精分化液较为重要，应至切片上的红色多余染液脱去，肌纤维呈鲜红色为止。
3、偶氮荧光桃红染色液为过饱和试剂，使用时吸取上清即可。
4、试剂开封后请尽快使用，以防影响后续实验效果。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Muscle fibers are a component of muscle tissue and are composed of muscle cells. According to their morphological and functional characteristics, muscle fibers can be classified into smooth muscle (also known as non-striated muscle), skeletal muscle, and cardiac muscle. There are various staining methods for muscle fibers, such as the Ponceau method, Aniline Blue method, Tungsten Phosphotungstic Acid Hematoxylin method, etc. The Tannic acid-Azo Phloxine method achieves differential staining through the sequential action of two acidic dyes.
Muscle Fiber Staining Solution (Puchtler Tannic Acid-Azo Phloxine method) is mainly composed of Hematoxylin Staining Solution, Tannic Acid Differentiation Solution, Phosphomolybdic Acid Differentiation Solution, and Azo Phloxine Staining Solution. Its staining principle is that tannic acid readily penetrates collagen fibers with high permeability, staining them yellow; Azo Phloxine easily enters muscle fibers with lower permeability, staining them red. This staining solution is used to distinguish muscle fibers from collagen fibers with clear contrast and resistance to fading. It can also demonstrate myoepithelial cells and can be used in the diagnosis of myoepithelial tumors of the breast, skin, and other tissues. This reagent is for research use only. Not for use in clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| M1518566 | Component | 5×50mL | Storage |
| M1518566A | Hematoxylin Staining Solution | 50mL | RT |
| M1518566B | Tannic Acid Differentiation Solution | 50mL | RT |
| M1518566C | Phosphomolybdic Acid Differentiation Solution | 50mL | RT . Store in the dark. |
| M1518566D | Azo Phloxine Staining Solution | 50mL | 2-8℃. Store in the dark. |
| M1518566E | Acid Alcohol Differentiation Solution | 50mL | RT |
Materials Required:
1. Bouin fixative, Carnoy fixative, neutral formalin, etc.
2. Distilled water, graded ethanol, xylene or eco-friendly dewaxing & clearing agent, neutral balsam
Protocol (For Reference Only):
1. Fix routine tissue specimens in Bouin fixative (Carnoy fixative or neutral formalin is also acceptable) for 2–3 hours.
2. Transfer directly into 95% ethanol for routine dehydration and embedding.
3. Prepare paraffin sections at 4 μm thickness; dewax with xylene or dewaxing & clearing agent and rehydrate to water.
4. Stain in hematoxylin staining solution for 8–10 minutes, then rinse in running water for 10 minutes.
5. Treat with tannic acid differentiation solution for 10 minutes, then rinse in running water for 1 minute.
6. Treat with phosphomolybdic acid differentiation solution for 10 minutes.
7. Stain in azo phloxine staining solution for 10–15 minutes.
8. Differentiate in acid alcohol differentiation solution for 3–5 seconds.
9. Dehydrate repeatedly in absolute ethanol, clear in xylene or dewaxing & clearing agent, and mount with neutral balsam.
Staining Results:
Muscle fibers, myoepithelial cells: Rose red
Collagen fibers: Yellow
Nuclei: Blue
Precautions:
1. Prefer Bouin fixative for tissue fixation; other fixatives may result in weaker staining.
2. Acid alcohol differentiation solution is critical. Differentiate until excess red dye is removed and muscle fibers appear bright red.
3. Azo phloxine staining solution is a supersaturated reagent. Use only the supernatant.
4. Use reagents promptly after opening to avoid affecting experimental results.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
肌纤维染色液
英文别名
Myofibre Staining Solution set (Puchtler's Tannic Acid Azo Fluorescent Myrtle Method)
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Muscle Fiber Staining Solution (Puchtler Tannic Acid-Azo Phloxine Method)
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0535345 |
分析证书 | M1518566 |
技术文档和文章
溶液计算器
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