计算溶液所需的质量、体积或浓度。
地衣红弹力纤维染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
弹力纤维(ElasticFiber)主要分布于人体的动脉壁、肺泡壁、皮肤，新鲜时呈黄色，折光性强。常用的弹力纤维染色法有Gomori醛品红法、间苯二酚碱性品红法、地衣红法、维多利亚蓝法、铁碘苏木素法等，弹力纤维染色可以显示皮肤组织中弹力纤维的变化，如弹力纤维痣、皮肤环状肉芽肿、硬度病等；显示与判定心内膜及动脉的病变，观察某些病变中是击伴有弹力纤维的增生或破坏；还可鉴别肿瘤组织成分如弹力纤维瘤，经弹力纤维染色后可清晰见到瘤体内弹力纤维球。
地衣红弹力纤维染色液操作简单、特异性高，但染色效果可能受到不同批次原料而有差异，有时会染色肝细胞内的脂褐素。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| L1518590 | Component | 3×50mL | Storage |
| L1518590A | Shikata 氧化剂 | 25mL | 2-8℃. Store in the dark. |
| L1518590B | Shikata 酸溶液 | 25mL | RT |
| L1518590C | Shikata 洗涤液 | 50mL | RT |
| L1518590D | 地衣红染色液 | 50mL | 2-8℃. Store in the dark. |
临用前，取A、B等量混合，即为Shikata 酸化液，即配即用。
自备材料：
10%中性福尔马林固定液、蒸馏水、系列乙醇、二甲苯或环保脱蜡透明液、中性树胶
操作步骤(仅供参考)：
1、组织固定于10%的中性福尔马林固定液，常规脱水包埋。
2、切片厚4～5μm，常规二甲苯或脱蜡脱蜡至水。
3、入Shikata酸化液氧化5min，稍水洗。
4、入Shikata洗涤液漂白2min，脱去酸化液的着色，流水冲洗2min。
5、入70%乙醇稍浸洗。
6、入地衣红染色液加盖浸染5h或更长时间。
7、常规脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
弹力纤维：棕红色至深棕色
背景：不同程度的灰棕色或淡红色
注意事项：
1、Shikata酸化液必须即配即用，混合后久置会降低其氧化力。
2、酸性地衣红染色液临用前应恢复至室温，染液可重复使用数次。
3、染色5h后，可取出切片在70%乙醇中浸洗一下，再入蒸馏水水洗，镜下观察。如染色较淡，可在70%乙醇中浸洗后入酸性地衣红染色液染色，必要时可过夜染色。
4、地衣红染色时，脂褐素和HBsAg阳性物质亦可称阳性反应。其区别方法是：脂褐素着色较深，在细胞质内呈大小均匀的小颗粒；HBsAg着色较浅，呈细微颗粒状；弹力纤维着色较深，不形成颗粒状。
5、试剂开封后请尽快使用，以防影响后续实验效果。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Elastic fibers are mainly distributed in the arterial wall, alveolar wall, and skin of the human body. They appear yellow when fresh and possess strong refraction. Common staining methods for elastic fibers include Gomori Aldehyde Fuchsin method, Resorcin Fuchsin method, Orcein method, Victoria Blue method, Iron Iodine Hematoxylin method, etc. Elastic fiber staining can demonstrate changes of elastic fibers in skin tissue, such as elastofibroma, annular granuloma of the skin, and scleroderma; it can reveal and evaluate endocardial and arterial lesions, and observe whether certain lesions are accompanied by hyperplasia or destruction of elastic fibers. It can also be used to identify tumor components such as elastofibroma, in which elastic fiber globules can be clearly seen after elastic fiber staining.
Lichen Red Elastic Fiber Staining Solution is easy to operate and highly specific, but the staining effect may vary with different batches of raw materials, and sometimes it may stain lipofuscin in liver cells. This reagent is for research use only. Not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| L1518590 | Component | 3×50mL | Storage |
| L1518590A | Shikata Oxidizer | 25mL | 2-8℃. Store in the dark. |
| L1518590B | Shikata Acid Solution | 25mL | RT |
| L1518590C | Shikata Washing Solution | 50mL | RT |
| L1518590D | Orcein Staining Solution | 50mL | 2-8℃. Store in the dark. |
Immediately before use, mix equal volumes of Solution A and Solution B to prepare Shikata Acid Solution. Prepare fresh and use immediately.
Materials Required:
10% neutral formalin fixative, distilled water, graded ethanol, xylene or eco-friendly dewaxing and clearing agent, neutral balsam
Protocol (For Reference Only):
1. Fix tissues in 10% neutral formalin fixative, then process for routine dehydration and embedding.
2. Cut sections at 4–5 μm thickness. Deparaffinize routinely with xylene or dewaxing agent and rehydrate to water.
3. Oxidize in Shikata Acid Solution for 5 min, then rinse briefly in water.
4. Bleach in Shikata Washing Solution for 2 min to remove color from the acid solution, then rinse in running water for 2 min.
5. Rinse briefly in 70% ethanol.
6. Stain covered in Orcein Staining Solution for 5 h or longer.
7. Dehydrate routinely, clear in xylene or dewaxing and clearing agent, and mount with neutral balsam.
Staining Results:
Elastic fibers: Brownish red to dark brown
Background: Grayish brown or pale pink to varying degrees
Precautions:
1. Shikata Acid Solution must be prepared fresh immediately before use. Prolonged standing after mixing will reduce its oxidizing activity.
2. Acid Orcein Staining Solution should be returned to room temperature before use. The staining solution can be reused several times.
3. After 5 h of staining, sections may be rinsed briefly in 70% ethanol, washed in distilled water, and examined microscopically. If staining is faint, sections may be rinsed in 70% ethanol and returned to Acid Orcein Staining Solution; overnight staining is acceptable if necessary.
4. Lipofuscin and HBsAg-positive substances may also stain positively with orcein. They can be distinguished as follows:
Lipofuscin: intensely stained, small granules of uniform size in the cytoplasm
HBsAg: weakly stained, fine granular pattern
Elastic fibers: intensely stained, non-granular
5. Use reagents promptly after opening to avoid affecting experimental results.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
地衣红弹力纤维染色试剂盒
英文别名
Lichen Red Elastane Dyeing Liquid set
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Lichen Red Elastic Fiber Staining Solution
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 F2617582 |
分析证书 | L1518590 |
| ZJ26F0535376 |
分析证书 | L1518590 |
技术文档和文章
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Application Differences Among Highman, Bennhold, Puchtler, and Modified Methods in Congo Red Staining for Amyloid
目录： 技术文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
Elastic Fiber Staining Methods: Comparison of Orcein, Verhoeff, Weigert, and Victoria Blue Methods
目录： 技术文章
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