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基因编辑
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FnCas12a (Cpf1)
- 种属: 土拉弗朗西斯菌亚种.诺维奇达(U112菌株)(Francisella tularensis subsp. novicida (strain U112))
- Accession #: A0Q7Q2.1
- 表达系统: E. coli
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Cas9 Nuclease (SpCas9)
- 种属: 化脓性链球菌血清型M1(Streptococcus pyogenes serotype M1)
- Accession #: Q99ZW2.1
- 表达系统: E. coli
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Cas9 NLS (SpCas9-NLS)
- 种属: 化脓性链球菌血清型M1(Streptococcus pyogenes serotype M1)
- Accession #: Q99ZW2.1
- 表达系统: E. coli
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T3 RNA 聚合酶
- 种属: 肠杆菌噬菌体T3(Enterobacteria phage T3)
- Accession #: YP_009792932.1,P07659
- 表达系统: E. coli
- 生物活性: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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牛痘DNA拓扑异构酶I
- 种属: 痘苗病毒(Vaccinia virus)
- Accession #: P68698.1
- 表达系统: E. coli
- 生物活性: One unit of DNA Topoisomerase I from Vaccinia converts 1 µg of supercoiled closed circular pUC18 DNA to relaxed closed circular form in 30 min at 37°C.
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AlkB (RNA/DNA 去甲基化酶, Nuclease-free)
- 种属: 大肠杆菌K12株(Escherichia coli (strain K12))
- Accession #: P05050.1
- 表达系统: E. coli
- 生物活性: One unit (U) is the amount of AlkB that removes 90-100% of the m6A modifications in total RNA of HEK 293T cells in 20 ul of reaction mixture at 37℃ after 100minutes of incubation. Under the regular conditions, the enzymatic activity of 1 μg of AlkB is approximately 1 unit.
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SP6 RNA 聚合酶
- 种属: 肠杆菌噬菌体SP6(Enterobacteria phage SP6)
- Accession #: Q7Y5N4
- 表达系统: E. coli
- 生物活性: One unit of enzyme incorporates 1nmol of AMP into polynucleotide in 1 hour at 37°C under the following activity assay conditions.
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T7 Endonuclease I
- 种属: Escherichia phage T7
- Accession #: P00641.1
- 表达系统: E. coli
- 生物活性: One unit is defined as the amount of enzyme required to convert > 90% of 1μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37℃.
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热敏感性双链脱氧核糖核酸酶
- 种属: Pandalus borealis
- Accession #: C9YSL6
- 表达系统: 毕赤酵母
