PEG8000 (50%, RNase free)

CAS: 25322-68-3 货号: P748949 分子式: HO(CH2CH2O)nH EC号: 500-038-2
有货
级别和纯度: BioReagent ? 生物试剂级(BioReagent)—— 经测试适用于生命科学和分子生物学。适用于需要生物相容性的细胞培养、检测和生化工作。 无DNA酶和RNA酶 ? 无 DNase 和 RNase 活性,保持 DNA 和 RNA 完整。适用于存在降解风险的核酸提取、PCR 和储存。 无蛋白酶 ? 无蛋白酶 —— 经验证无蛋白酶活性,保护蛋白质和酶。适用于须避免蛋白水解的蛋白制备和检测。 ≥99% 50%
别名
聚乙二醇8000
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
★
规格
库存
价格
数量
2ml
P748949-2ml
现货 Stock Image
¥99.90
请输入您要添加的产品数量
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为什么选择此级别

BioReagent,无DNA酶和RNA酶,无蛋白酶 级 提供 ≥99% 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

📋

质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 3253 篇同行评审文献引用。

概述

本公司生产的 PEG8000 (50%, RNase free)是一种可以用于 RNA、DNA 等分子生物学实验的无 RNA 酶、无 DNA 酶并且也没有蛋白酶污染的 50%聚乙二 醇 8000 溶液。本产品主要用于涉及 RNA 或 DNA 的分子生物学实验等,例如用于 RNA 或 DNA 的连接反应以增强连接效率等。PEG8000 即 Polyethylene Glycol 8000,中文名为聚乙二醇 8000。分子式为 H (OCH₂CH₂)nOH,分子量为 7000-9000,纯度>99%。 

产品应用:

用于 T4 RNA Ligase 1 (ssRNA Ligase)催化单链 RNA 或 DNA 的分子间连接反应;T4 RNA Ligase 2 (dsRNA Ligase)催化 RNA/DNA 杂合链中 RNA 3' 羟基与 DNA 5'磷酸基的缺刻连接反应;T4 RNA Ligase 2, truncated 催化 5'端腺苷酰化 DNA (AppDNA)和单链 RNA (ssRNA)的 3'羟基末端连接反应; 以及小 RNA 3'接头及连接试剂盒催化 5'端腺苷酰化的 Universal miRNA Cloning Linker 和单链 RNA (ssRNA) 3'羟基末端的连接反应。 

产品优势 

高纯度和无 RNase 污染,高效沉淀核酸,适用于多种核酸提取方法。 

注意事项: 

(1)本产品为粘稠溶液,使用时完全混合均匀后再吸取,且保证吸取体积准确。 

(2)本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。 

(3)为了您的安全和健康,请穿实验服并戴一次性手套操作。

(4)本品在-20℃冷冻保存后,因低温诱导产生“冷冻-浓缩效应”,溶液可能会凝固为白色胶冻状或絮状沉淀。此为PEG8000高浓度溶液的固有物理特性,属正常现象,不影响产品质量。若出现这种现象, 将整管产品置于37℃恒温水浴中加热10–20分钟,期间每隔5分钟轻轻颠倒或涡旋混匀,直至胶冻状物质完全消失,溶液恢复为透明粘稠液体,即可正常使用。

Our company’s PEG8000 (50%, RNase Free) is a 50% polyethylene glycol 8000 solution free of RNase, DNase and protease contaminants, suitable for molecular biology experiments involving RNA, DNA and other nucleic acids. This product is primarily applied in molecular biology assays with RNA or DNA, such as ligation reactions of RNA or DNA to improve ligation efficiency. PEG8000 stands for Polyethylene Glycol 8000, with the Chinese name polyethylene glycol 8000. Its molecular formula is H(OCH₂CH₂)ₙOH, molecular weight ranges from 7000 to 9000, and purity exceeds 99%.

Product Applications

1. Intermolecular ligation of single-stranded RNA or DNA catalyzed by T4 RNA Ligase 1 (ssRNA Ligase);

2. Nick ligation between the 3'-hydroxyl of RNA and 5'-phosphate of DNA within RNA/DNA hybrid strands catalyzed by T4 RNA Ligase 2 (dsRNA Ligase);

3. Ligation between 5'-adenylylated DNA (AppDNA) and the 3'-hydroxyl terminus of single-stranded RNA (ssRNA) catalyzed by truncated T4 RNA Ligase 2;

4. Ligation between 5'-adenylylated Universal miRNA Cloning Linker and the 3'-hydroxyl terminus of single-stranded RNA (ssRNA) using small RNA 3' Adapter Ligation Kit.

Product Advantages

High purity, RNase-free, efficient nucleic acid precipitation, compatible with various nucleic acid extraction protocols.

Precautions

1. This product is a viscous solution. Thoroughly homogenize the solution before pipetting to ensure accurate volume transfer.

2. For research use only by professional researchers. Not for clinical diagnosis or therapy, food or pharmaceutical manufacturing. Do not store in residential premises.

3. Wear a lab coat and disposable gloves during handling for personal safety and health protection.

规格

别名
聚乙二醇8000
英文别名
Polyethylene Glycol 8000
规格或纯度
BioReagent, 无DNA酶和RNA酶, 无蛋白酶, ≥99%, 50%
稳定性与储存
长期储存-20℃(24个月),避免反复冻融。
英文名称
PEG8000 (50%, RNase free)
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
纯度
≥99%
产品线
biological_products
名称和识别符
EC号
500-038-2
分子类型
聚合物
Reaxy-Rn
505945
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=505945&ln=

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

化学和物理性质
折光率
1.460
闪点(℉)
519.8 °F
闪点(℃)
271.00 °C
沸点
> 200 °C
熔点
61-64℃
安全和危险性(GHS)
WGK Germany
1
Merck Index
7568
个人防护装备
Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到3个结果

批号(Lot Number) 证书类型 货号
ZJ25F1230595 分析证书 P748949
ZJ26F0737412 分析证书 P748949
ZJ25F0724919 分析证书 P748949
技术文档和文章
无蛋白酶级
Protease Free Grade
无DNA酶和RNA酶级试剂:概述
DNase/RNase-Free Grade Reagents: Overview
无蛋白酶级
Protease-Free Grade
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Enzyme Protectants and Common Methods to Prolong Enzymatic Activity
如何使用透析袋对样品进行缓冲液置换
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为什么含生物素的多肽在生物素检测中会呈阴性?
Why Can Biotinylated Peptides Test Negative in Biotin Assays?
如何选择血浆游离DNA的提取试剂
How to Choose Plasma Cell-Free DNA Extraction Reagents
转化与转染的区别
Differences Between Transformation and Transfection
PEG化技术在药物修饰与递送中的应用:蛋白、多肽、小分子及脂质体
PEGylation in Drug Modification and Delivery: Applications to Proteins, Peptides, Small Molecules and Liposomes
聚乙二醇(PEG)的性质、多领域应用及产品选型指南
Polyethylene Glycol (PEG): Properties, Applications Across Multiple Fields, and Product Selection Guide
PEG–透析袋法蛋白质浓缩
PEG–Dialysis Tubing Method for Protein Concentration
Super Fluor 680 / 750 近红外染料在小动物活体荧光成像中的应用指南
Application Guide to Super Fluor 680 / 750 Near-Infrared Dyes for In Vivo Small-Animal Fluorescence Imaging
7种DNA连接酶的作用及特点
7 Types of DNA Ligases: Functions and Features
NHS 活化酯抗体标记的通用原理、F/P 计算与 Troubleshooting 指南
General Principles of Antibody Labeling with NHS-Activated Esters, F/P Calculation, and Troubleshooting Guide
聚乙二醇(PEG)及其衍生物:端基设计与在 PROTAC、ADC 和医疗器械中的应用
Polyethylene Glycol (PEG) and Its Derivatives: End-Group Design and Applications in PROTACs, ADCs, and Medical Device
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Sources, Biological Functions, Isolation/Characterization, and Emerging Applications of Exosomes
PEG 基水凝胶光交联与图案化(2D/3D):原理、方法对比与产品选型指引
Photo-Crosslinking and Patterning of PEG-Based Hydrogels (2D/3D): Principles, Method Comparisons, and Product Selection Guidelines
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此产品的引用文献
溶液计算器