Introduction: Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10⁻¹⁴ mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Cy5 conjugated streptavidin can be used to localize or to detect biotinylated biomolecules in cells, tissue sections or other fluorescent immunoassays. Application: Flow: 0.02-5μg/mL IF: 1:1000 WB: 1:5000-1:80000 Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Recombinant Streptavidin Protein (Cy5) (rp169047) - IF IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Biotin) (Ab175850) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/2000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (Cy5) (rp169047) - Flow Cytometry BALB/c mouse splenocytes stained with Biotin-CD45R antibody, followed by Recombinant Streptavidin Protein (Cy5) (rp169047) at 0.04 µg/test concentration.
Recombinant Streptavidin Protein (Cy5) (rp169047) - Flow Cytometry BALB/c mouse splenocytes stained with Biotin-CD45R antibody for 1 hour at 4°C, followed by 0.04 μg Recombinant Streptavidin Protein (Cy5) (rp169047) (red line) for 30 minutes at 4°C in the dark. Unlabeled samples (black line) were used as a negative control.
Recombinant Streptavidin Protein (Cy5) (rp169047) - Flow Cytometry Flow analysis of EGF receptors (red line) in A431 Cells. Cells were labeled with Recombinant Human EGF Protein (Biotin) (rp170191) at 1/2500 dilution for 1 hour at 4°C, and then incubated with Recombinant Streptavidin Protein (Cy5) (rp169047) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Unlabeled Cells (black line) were used as a negative control.
ZJ25F0723653
危险品化学品经营许可证（带存储）