Introduction: Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10⁻¹⁴ mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Streptavidin-PerCP is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. Recommended Usage: Flow: 0.05-2.5 μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
PBS (pH 7.4) buffer containing 1% BSA and 0.05% ProClin300
防腐剂
0.05% ProClin300
浓度
0.2 mg/mL
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
4℃ 下运输。未经稀释,储存于 4℃,避免长时间暴露于光线下。请勿冷冻。
图片
Recombinant Streptavidin Protein (PerCP) (rp169052) - Flow Cytometry BALB/c mouse splenocytes stained with Biotin-CD45R antibody, followed by Recombinant Streptavidin Protein (PerCP) (rp169052) at 0.06 μg/test concentration.
Recombinant Streptavidin Protein (PerCP) (rp169052) - Flow Cytometry BALB/c mouse splenocytes stained with Biotin-CD45R antibody for 1 hour at 4°C, followed by 0.06 μg Recombinant Streptavidin Protein (PerCP) (rp169052) (red line) for 30 minutes at 4°C in the dark. Unlabeled samples (black line) were used as a negative control.