Application： Flow: 0.05-5μg/mL IF: 1-10μg/mL Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
保存在 -20°C 温度下。避光保存。避免冷冻/解冻循环。
图片
Recombinant Streptavidin Protein (AF647) (rp181946)- IF IF analysis of beta Tubulin (red) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with biotinylated beta Tubulin Mouse mAb (Ab179922) dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Streptavidin Protein (AF647) (rp181946) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (AF647) (rp181946) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF647) (rp181946) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ24F0606582
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