Em:660nm,Ex:650nm,无叠氮钠,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 73 篇同行评审文献引用。
Introduction:
Streptavidin is a tetrameric protein purified from Streptomyces avidinii that binds very tightly to the vitamin biotin with a dissociation constant on the order of ≈10⁻¹⁴ mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry, microplate assays and microarrays.
Conjugation:
The APC was conjugated with Streptavidin under optimum conditions. Unconjugated streptavidin and mostly unconjugated APC were removed. The APC conjugates are prepared from chemically crosslinked APC to avoid dissociation of the molecule into subunits when highly diluted.
Recommended Usage for Application:
Flow: 0.02-1.25μg/mL
Application Note:
Centrifuge protein conjugate solutions briefly in a microcentrifuge before use and use only the supernatant for the experiment. This step will eliminate any protein aggregates that may have formed in solution, thereby reducing nonspecific background staining.
Recombinant Streptavidin Protein (APC) (rp169048) - Flow Cytometry
BALB/c mouse splenocytes stained with Biotin-CD45R antibody, followed by Recombinant Streptavidin Protein (APC) (rp169048) at 0.03 μg/test concentration.
Recombinant Streptavidin Protein (APC) (rp169048) - Flow Cytometry
BALB/c mouse splenocytes stained with Biotin-CD45R antibody for 1 hour at 4°C, followed by 0.03 μg Recombinant Streptavidin Protein (APC) (rp169048) (red line) for 30 minutes at 4°C in the dark . Unlabeled samples (black line) were used as a negative control.
0.02-1.25 μg/mL
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F1039918 |
分析证书 | rp169048 |
| ZJ26F1039917 |
分析证书 | rp169048 |
| ZJ26F1039916 |
分析证书 | rp169048 |
| ZJ23F1001378 |
分析证书 | rp169048 |
| ZJ23F1001377 |
分析证书 | rp169048 |
| ZJ25F0319660 |
分析证书 | rp169048 |
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