Application: ELISA: 1:1000-1:20000 WB: 1:1000-1:20000 Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
保存在 -20°C 温度下。避光保存。避免冷冻/解冻循环。
图片
Recombinant Streptavidin Protein (alkaline phosphatase) (np169050) - Western Blot All lanes: beta Actin Mouse mAb (Biotin) (Ab175850) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Recombinant Streptavidin Protein (alkaline phosphatase) (np169050) at 1/10000 dilution Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature. Predicted band size: 42 kDa Observed band size: 42 kDa
Recombinant Streptavidin Protein (alkaline phosphatase) (np169050) - ELISA Recombinant Streptavidin Protein (alkaline phosphatase) (np169050) was tested by ELISA. The plate was coated with 1.0 µg/mL (100 µL/well) Mouse IgG (Ab170221). Then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002). A serial dilution of Recombinant Streptavidin Protein (alkaline phosphatase) (np169050) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 1hour incubated at room temperature.