SA V1 | SA V2 | strepavidin | Streptavidin V1 | Streptavidin V2 | Streptavidin-AF594 | AF594-Streptavidin | SA-AF594
规格或纯度
ExactAb™, 已验证, Ex:590nm, Em:618nm, 1.0 mg/mL
生物活性
Binding Biotin
表达系统
E. coli
种属
阿维迪尼链霉菌(Streptomyces avidinii)
无载体
No
无动物源
No
偶联
AF594
Excitation(nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
来源
重组表达
预测分子量
19 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% ProClin300
浓度
1.0 mg/mL
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The product should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant Streptavidin Protein (AF594) (rp181945) - IF IF analysis of beta Tubulin (red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Recombinant Streptavidin Protein (AF594) (rp181945) - Flow Cytometry Flow cytometry analysis of CD45 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD45 Mouse mAb (Biotin) (Ab179532) at 0.05μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (AF594) (rp181945) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
IF/ICC
1:500
Flow Cytometry
≤1.0 µg/test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. Since applications vary, you should determine the optimum working
ZJ24F1215850
危险品化学品经营许可证（带存储）