概述

磷酸盐缓冲盐溶液(PBS，Phosphate Buffered Solution)是一种平衡盐溶液，用于各种细胞培养应用，如消化前清洗细胞、运输细胞或组织样品、稀释细胞进行计数和制备试剂。PBS不含钙和镁，用于在细胞解离前从培养物中冲洗螯合剂。

本品在传统配方上进行改良，去除了HCl调节pH过程，从而避免了因HCl引入带来的渗透压、pH、缓冲曲线等参数的不稳定，有效改善因不稳定因素在长期细胞培养过程中对细胞的刺激积累造成的种系突变等问题。
成份：Sodium phosphate dibasic；Potassium Phosphate Monobasic；Sodium chloride

使用方法：
1.使用三级水或一级水溶解本品并定容至200ml，可得200ml体积的10X磷酸盐缓冲盐溶液。2.若需配置1X浓度磷酸盐缓冲盐溶液，可用三级水或一级水溶解本品并定容至2L，或将10X浓缩液与三级水或一级水按1:9比例进行稀释。3.溶解后所得磷酸盐缓冲盐溶液经0.22μm过滤除菌后，可用于细胞生物学实验，配制用水内毒素需低于0.01EU/ml并用去内毒素的无菌容器盛装。4.蒸气灭菌会导致溶液体积损失并造成溶液浓度,pH,渗透压等参数变化，请勿采用蒸气灭菌方法对溶液进行除菌。5.本品溶解后pH在25℃-37℃可稳定于所标注的范围内，无需使用HCl进一步进行调节，如需其他pH的磷酸盐缓冲盐溶液，可购买对应产品或进行定制。

适用范围：

适用于一般生物学及医学研究

Phosphate Buffered Solution (PBS) is a balanced saline solution used in various cell culture applications, such as pre-digestion cell washing, transportation of cells or tissue samples, cell dilution for counting, and reagent preparation. PBS lacks calcium and magnesium and is used to flush out chelating agents from cultures prior to cell dissociation.

This product is an improvement over the traditional formulation, eliminating the HCl step for pH adjustment. This modification avoids instability in osmotic pressure, pH, and buffer curves caused by HCl introduction, effectively mitigating issues such as germline mutations resulting from cumulative cellular stimulation due to unstable factors during long-term cell culture.

Ingredients:Sodium phosphate dibasic；Potassium Phosphate Monobasic；Sodium chloride

Usage method

1. Dissolve this product in water of grade III or grade I and adjust the volume to 200 mL to obtain a 200 mL volume of 10X phosphate buffered saline solution.2. For preparing a 1X phosphate buffer saline solution, dissolve the product in deionized water (DW) or ultrapure water (UPW) and adjust the volume to 2L, or dilute the 10X concentrate with DW or UPW in a 1:9 ratio.3. The phosphate buffer saline solution obtained after dissolution shall be filtered through a 0.22 μm filter for sterilization before being used in cell biology experiments. The endotoxin concentration in the preparation water must be below 0.01 EU/ml, and the solution shall be stored in sterile containers with endotoxin removal.4. Steam sterilization may lead to volume loss of the solution and cause changes in parameters such as solution concentration, pH, and osmotic pressure. Do not use steam sterilization for solution sterilization.5. The pH of this product remains stable within the labeled range after dissolution at 25°C-37°C without further adjustment with HCl. For phosphate buffer saline solutions requiring other pH values, corresponding products may be purchased or customized.

规格

规格或纯度

1×,pH7.2-7.4,,粉剂,不含Ca2+/Mg2+

英文名称

Phosphate Buffered Solution

储存条件

室温

运输条件

常规运输

名称和识别符

分子类型

未知

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产品属性

pH	7.2-7.4

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批号(Lot Number)	证书类型	货号
D2627449	分析证书	P301982
K2521085	分析证书	P301982
C2317688	分析证书	P301982
K2219001	分析证书	P301982
D2208007	分析证书	P301982
J2127774	分析证书	P301982