1. Immobilized Recombinant Human IL-6 Protein at 2 μg/mL can bind Recombinant Human IL-6R Protein (rp147628) the EC50 is 8.0 - 24.0 ng/mL. 2. Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 10.0 - 60.0 ng/mL.3. Using the Octet RED System, the affinity constant (Kd) of Recombinant Human IL-6R Protein (rp147628) bound Anti-IL6R Antibody was 0.1 nM.
Function: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
1. Immobilized Recombinant Human IL-6 Protein at 2 μg/mL can bind Recombinant Human IL-6R Protein (rp147628) the EC50 is 8.0 - 24.0 ng/mL. 2. Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 10.0 - 60.0 ng/mL.3. Using the Octet RED System, the affinity constant (Kd) of Recombinant Human IL-6R Protein (rp147628) bound Anti-IL6R Antibody was 0.1 nM.
Recombinant Human IL-6R Protein (rp147628) - Protein Bioactivity Immobilized Recombinant Human IL-6 Protein at 2 μg/mL can bind Recombinant Human IL-6R Protein (rp147628) the EC₅₀ is 8.0 - 24.0 ng/mL.
Recombinant Human IL-6R Protein (rp147628) - Protein Bioactivity Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED₅₀ for this effect is typically 10.0 - 60.0 ng/mL.
Recombinant Human IL-6R Protein (rp147628) - BLI Assay Using the Octet RED System, the affinity constant (Kd) of Recombinant Human IL-6R Protein (rp147628) bound Anti-IL6R Antibody was 0.1 nM.
Recombinant Human IL-6R Protein (rp147628) - SDS-PAGE 3 μg/lane of Recombinant Human IL-6R Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 71.1 kDa under reducing conditions and 68.4 kDa under non-reducing conditions.