CDHF12MTC1 | CDHR16 | EC 2.7.10 | Hirschsprung disease 1 | Hirschsprung disease) | MEN2A | MEN2B | multiple endocrine neoplasia and medullary thyroid carcinoma 1 | ret proto-oncogene | RET transforming sequence | Ret
规格或纯度
无动物源, 无载体, His标签, PBS Only, ≥90%(SDS-PAGE), 见COA
生化机理
Receptor tyrosine-protein kinase, also known as Ret, is encoded by the C-RET proto-oncogene. Mutations of the C-RET gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung's disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human C-RET cDNA encodes a 1115 amino acid (aa) protein with a 28 aa signal peptide, a 679 aa cysteine-rich extracellular domain that contains a membrane-anchored Ret-Cadherin 120 Da fragment, and a 456 aa cytoplasmic domain with a soluble Ret kinase fragment. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha.
Post-translational: Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.
94.6-126.7 kDa, under reducing conditions; 93.4-121.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。
图片
Recombinant Human Ret Protein (rp188327) - SDS-PAGE 2 μg/lane of Recombinant Human Ret Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 94.6-126.7 kDa under reducing conditions and 93.4-121.8 kDa under non-reducing conditions.
危险品化学品经营许可证（带存储）