ExactAb™,已验证,重组 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Rabbit anti Human JNK1/JNK2/JNK3 Antibody, Recombinant, could be used for WB, ICC, IF, IP and so on.
Application:
WB: 1/1000-1/2000
ICC/IF: 1/50-1/200
IP: 1/50
Protein Function：
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - Western Blot
All lanes: Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 46, 54 kDa
Observed band size: 39, 46 kDa
Exposure time: 100.0 s
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - IP
Immunoprecipitate (IP) analysis using Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/50 dilution. (WB with Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/3000 dilution).
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) at 1/50 dilution.
Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant JNK1/JNK2/JNK3 Antibody (Ab111513).
1/50-1/200
1/50
1/1000 - 1/2000
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1012514 |
分析证书 | Ab111513 |
| ZJ24F1012515 |
分析证书 | Ab111513 |
| ZJ26F0435046 |
分析证书 | Ab111513 |
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