Rabbit anti Human MCM7/PRL Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/200
Protein Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Recombinant MCM7/PRL Antibody (Ab114573) - Western Blot All lanes: Recombinant MCM7/PRL Antibody (Ab114573) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 81 kDa Observed band size: 82 kDa Exposure time: 8.8 s
Recombinant MCM7/PRL Antibody (Ab114573) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant MCM/7PRL Antibody (Ab114573). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant MCM7/PRL Antibody (Ab114573) - IF/ICC
IF analysis of Recombinant MCM7/PRL Antibody (Ab114573) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MCM7/PRL Antibody (Ab114573) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
ZJ23F0901144
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