Rabbit anti Human SHP2 Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
IF/ICC: 1/500
Protein Function:
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Recombinant SHP2 Antibody (Ab127519) - Western Blot All lanes: Recombinant SHP2 Antibody (Ab127519) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 68 kDa Observed band size: 67 kDa Exposure time: 30.0 s
Recombinant SHP2 Antibody (Ab127519) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant SHP2 Antibody (Ab127519). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant SHP2 Antibody (Ab127519) - IF/ICC
IF analysis of Recombinant SHP2 Antibody (Ab127519) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SHP2 Antibody (Ab127519) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
ZJ24F0403984
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