中性树胶是显微技术上使用的优良封藏剂，与加拿大树胶性质相似，用途相同，效果较好。中性树胶是一种天然树脂经提炼与中和后溶解在二甲苯中形成60%的溶液，折光率与玻璃近似，干燥后凝结成透明无色的固体，没有收缩变黄、纹裂的弊病，没有像加拿大树胶有年久变黄、切片氧化褪色等缺点。苏木精、固绿等染色的切片标本保藏数年无颜色变化。许多整装标本如吸血虫、蚜虫等寄生虫，用中性树胶封固，均能取得优良效果。目前，中性树胶广泛使用在生物学、病理学等作为生物切片的封藏剂，以及光学玻璃仪器的粘固剂。
环保封片胶由树脂、替代二甲苯的有机溶剂等配制而成，因不含有毒有害的二甲苯，对环境友好，是优良的中性树胶的替代产品。环保封片胶可用于染色后的封片，能使染色后的组织、细胞固封于载玻片和盖玻片之间，不与空气直接接触，避免氧化褪色；可使组织片、细胞片在封片胶的充实下，其折光率能与玻片的折光率相近，从而使镜检效果更清晰。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
1、切片染色完成后，吸去染色液，梯度乙醇脱水，二甲苯透明。
2、封片时，根据组织大小滴加0.5-1滴在标本正中间 (注意要滴在一起，否则容易有气泡)，轻轻盖上盖玻片，待胶液扩散满整个盖玻片即可。
注意事项：
1、荧光染色的切片不宜用中性树胶封片。
2、组织切片或细胞涂片等样本经染色和封片后，应及时镜检拍照。
3、封片时应注意通风，避免接触皮肤、黏膜。
4、使用前不宜搅拌或晃动，避免封片产生气泡。
5、每次使用后及时拧紧瓶盖，以免挥发或变质。在有效期内，开封后1年内使用完。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
Neutral balsam is an excellent mounting medium used in microscopy techniques. It is similar in properties and applications to Canada balsam, with superior performance. Neutral balsam is a 60% solution prepared by refining, neutralizing natural resins and dissolving them in xylene. Its refractive index is close to that of glass, and it solidifies into a transparent, colorless mass after drying without defects such as shrinkage, yellowing or cracking. Unlike Canada balsam, it does not cause yellowing over time or oxidative fading of sections. Tissue sections stained with hematoxylin, fast green, and other dyes can be preserved for several years without color change. It also yields excellent results when used for mounting whole-mount specimens such as parasites including schistosomes and aphids. Currently, neutral balsam is widely used as a mounting medium for biological sections in fields like biology and pathology, as well as an adhesive for optical glass instruments.
Eco-friendly mounting medium is formulated with resins and organic solvents that replace xylene. Free of toxic and harmful xylene, it is environmentally friendly and serves as an ideal alternative to neutral balsam. This medium can be used for mounting stained sections, sealing stained tissues and cells between slides and coverslips to prevent direct contact with air and avoid oxidative fading. Filled with the mounting medium, tissue and cell sections achieve a refractive index close to that of glass slides, resulting in clearer microscopic observation. This reagent is intended for research use only and not for clinical diagnosis or other purposes.
Operating Procedures (For Reference Only)
1. After completing section staining, aspirate the staining solution, dehydrate the sections through a graded ethanol series, and clear them with xylene.
2. For mounting, add 0.5-1 drop of the medium exactly in the center of the specimen (ensure the drop is placed as a single blob to avoid air bubbles). Gently place a coverslip over the specimen and wait until the medium spreads evenly across the entire coverslip surface.
Precautions
1. Neutral balsam is not suitable for mounting fluorescence-stained sections.
2. Stained and mounted specimens including tissue sections and cell smears should be examined under a microscope and photographed promptly.
3. Ensure adequate ventilation during the mounting process. Avoid contact with skin and mucous membranes.
4. Do not stir or shake the medium before use to prevent air bubbles from forming during mounting.
5. Tighten the bottle cap immediately after each use to prevent volatilization or deterioration. The opened reagent should be used up within 1 year, provided it is within the expiration date.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
7. Use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.
ZJ26F1039690
危险品化学品经营许可证（带存储）