增强型荧光淬灭封片剂(Enhanced Antifade Mounting Medium)属于抗荧光衰减封片剂(Fluorescence decay resistant Medium)的一种,是一种用于减缓荧光淬灭的封固剂,可以用于减缓各种常见荧光染料的荧光淬灭,较普通抗荧光淬灭封片剂的抗淬灭效果大大增强,一般1~2周后才会有所减弱,按每个样本需要50~100μl封片剂计算,5ml可以用于至少50样本的封片。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
自备材料:
1、载玻片
2、盖玻片
3、荧光显微镜
操作步骤(仅供参考):
1、贴壁细胞样本
（1）染色完成后,吸去染色液。
（2）滴1滴 Enhanced Fluorescent Mounting Medium 于载玻片上,盖上贴有细胞的盖玻片,让细胞接触封片液,尽量避免气泡。
（3）在荧光显微镜下观察或4℃避光保存1~2周。
2、组织切片
（1）染色完成后,吸去染色液。
（2）滴1滴Enhanced Fluorescent Mounting Medium于组织切片上,盖上盖玻片,让切片接触封片液,尽量避免气泡。
（3）在荧光显微镜下观察组织切片或4℃避光保存1~2周。
3、其它样品
其它样品参考细胞样品或组织切片进行操作。
注意事项:
1、抗荧光淬灭的概念是相对的,样本染色后最好立即观察,如果没有条件,最好应在当天观察。
2、荧光物质均易发生淬灭,染色后的样品宜4℃避光保存。
3、在使用Enhanced Fluorescent Mounting Medium的情况下,虽然可减缓淬灭,仍需尽量避光。
4、用罗丹明标记的抗体不能用该封片剂封片,可以采用聚乙烯醇荧光封片剂。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用,以防影响后续实验效果。
Enhanced Antifade Mounting Medium is a type of fluorescence decay-resistant mounting medium designed to slow down fluorescence quenching. It effectively suppresses quenching of all common fluorescent dyes and delivers far superior anti-quenching performance compared with standard antifade mounting media; its protective effect only slightly diminishes after 1 to 2 weeks. Calculated based on a usage volume of 50–100 μL per sample, a 5 mL bottle is sufficient for mounting at least 50 samples. This reagent is for research use only and shall not be applied for clinical diagnosis or other purposes.
Materials to Be Prepared Separately:
1. Glass slides
2. Coverslips
3. Fluorescence microscope
Operating Procedures (For Reference Only):
1. Adherent Cell Samples
(1) Aspirate staining solution after staining is completed.
(2) Drop one drop of Enhanced Antifade Mounting Medium onto the glass slide, then place the cell-attached coverslip over it to ensure full contact between cells and mounting medium, and minimize air bubbles.
(3) Observe under a fluorescence microscope, or store the slide at 4 °C protected from light for 1–2 weeks.
2. Tissue Sections
(1) Aspirate staining solution after staining is completed.
(2) Drop one drop of Enhanced Antifade Mounting Medium onto the tissue section, cover with a coverslip to ensure full contact between the section and mounting medium, and minimize air bubbles.
(3) Examine the tissue section under a fluorescence microscope, or store at 4 °C away from light for 1–2 weeks.
3. Other Specimens
Handle other specimens following the protocols for cell samples or tissue sections accordingly.
Precautions:
1. Anti-quenching performance is relative. Samples should ideally be observed immediately after staining; if immediate observation is unavailable, examine them on the same day.
2. Fluorescent substances are susceptible to quenching. All stained samples should be stored at 4 °C protected from light.
3. Although this medium slows fluorescence quenching, samples still require strict light avoidance after mounting.
4. Do not use this mounting medium for slides stained with rhodamine-conjugated antibodies; polyvinyl alcohol-based fluorescent mounting medium is recommended as an alternative.
5. Wear a lab coat and disposable gloves throughout all operations for personal safety and health protection.
6. Use up the reagent promptly once opened to avoid compromised experimental results.
ZJ26F0738073
危险品化学品经营许可证（带存储）