计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
微管相关蛋白轻链3B(LC3B)又称为自噬相关蛋白LC3B,是一种蛋白质,由LC3B基因编码。它是高度保守的ATG8蛋白家族的一个成员。ATG8蛋白存在于所有已知的真核生物体中。动物ATG8家族包括三个亚家族:微管相关蛋白1轻链3(MAP1LC3);16kDa的高尔基相关ATP酶增强剂(GATE-16)和γ-氨基丁酸受体相关蛋白(GABARAP)。它是MAP1LC3亚家族中的四个基因之一(其他包括MAP1LC3A、MAP1LC3C和LC3B2)。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人微管相关蛋白轻链3B(LC3B)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人微管相关蛋白轻链3B(LC3B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LC3B. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LC3B present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.