BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乳酸脱氢酶(lactate dehydrogenase，LDH或LD)属于氧化还原酶，能够催化氢氧原子或电子从一中底物转移到另一种底物上乳酸脱氢酶是糖酵解和糖异生的一个极其重要的酶，含有锌离子，广泛分布于人和动物组织、植物和微生物中，能可逆的催化乳酸(L)和丙酮酸(P)之间的氧化还原反应。其反应公式：乳酸+NAD⁺→丙酮酸+NADH+H⁺。L→P为正向反应；P→L为逆向反应。 该试剂盒是利用乳酸脱氢酶催化上述逆反应，即丙酮酸+NADH+H⁺→乳酸+NAD⁺，在上述反应过程中丙酮酸还原成乳酸，同时NADH氧化成NAD⁺，引起340nm处吸光度的下降，其下降速率与标品中LDH活性呈正比关系，通过分光光度计或自动分析仪检测340nm处吸光度下降速率，通过计算获得乳酸脱氢酶的活性；该LD-P法的优点是：1、操作比二硝基苯肼比色法简单；2、重复性好；3、准确性比二硝基苯肼法好；4、适用于自动分析仪。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
Lactate Dehydrogenase (LDH or LD) is an oxidoreductase enzyme that catalyzes the transfer of hydrogen/oxygen atoms or electrons from one substrate to another. It is an extremely important enzyme in glycolysis and gluconeogenesis, contains zinc ions, and is widely distributed in human and animal tissues, plants, and microorganisms. It reversibly catalyzes the oxidation-reduction reaction between lactate (L) and pyruvate (P). The reaction formula is: Lactate + NAD⁺ ↔ Pyruvate + NADH + H⁺. The L→P reaction is the forward reaction, while the P→L reaction is the reverse reaction. This kit utilizes lactate dehydrogenase to catalyze the reverse reaction mentioned above, namely: Pyruvate + NADH + H⁺ → Lactate + NAD⁺. In this reaction process, pyruvate is reduced to lactate, while NADH is oxidized to NAD⁺, causing a decrease in absorbance at 340 nm. The rate of this decrease is proportional to the LDH activity in the sample. The rate of absorbance decrease at 340 nm is measured using a spectrophotometer or an automated analyzer, and the lactate dehydrogenase activity is calculated accordingly. The advantages of this LD-P method are: 1. Simpler operation compared to the dinitrophenylhydrazine colorimetric method; 2. Good reproducibility; 3. Better accuracy than the dinitrophenylhydrazine method; 4. Suitable for use with automated analyzers. This kit is intended for research use only and is not for use in diagnostic procedures.
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