Name: α-凝血酶
SKU: np330890
Availability: InStock

α-凝血酶

CAS: 9002-04-4 货号: np330890 分子式: C₁₂H₁₀ClN₃S 分子量: 263.75 EC号: 232-648-7 PubChem CID: 65045

概述规格储存与运输高级数据产品属性质检证书(CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

概述

The Human Thrombin was activated from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. These activating enzymes were removed after activation. Complete activation was observed on SDS-PAGE. This activated enzyme has a minimum activity of 2,700 NIH units/mg when compared to NIH standard thrombin.

Extinction Coefficient (1%) = 18.3

The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.

Protein is for research use only; not for human use.

规格

产品名称

α-凝血酶

别名

凝血因子 Ⅱa

英文别名

规格或纯度

生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, 来源于人血浆; ≥2700 NIH U/mg protein; 冻干前浓度：见COA

生化机理

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Activity regulation: Activity is promoted in the presence of negatively charged surfaces, such as polyphosphate and dextran sulfate. Inhibited by SERPINA5. Post-translational: The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). In the penultimate step of the coagulation cascade, prothrombin is converted to thrombin by the prothrombinase complex composed of factor Xa (F10), cofactor Va (F5), and phospholipids. This activation requires factor Xa-catalyzed sequential cleavage at 2 sites, Arg-314 and Arg-363, along 2 possible pathways. In the first pathway, the first cleavage occurs at Arg-314, leading to the formation of the inactive intermediate prethrombin-2. This pathway preferentially occurs on platelets and in the absence of cofactor Va. In the second pathway, the first cleavage occurs at Arg-363, which separates protease domain into 2 chains that remain connected through a disulfide bond and generates the active intermediate meizothrombin. The presence of cofactor Va directs activation along the meizothrombin pathway and greatly accelerates the rate of cleavage at Arg-363, but has a smaller effect on the cleavage of meizothrombin at Arg-314. Meizothrombin accumulates as an intermediate when prothrombinase is assembled on the membrane of red blood cells.

生物活性

≥2700 NIH U/mg protein

种属

人(Human)

来源

天然

预测分子量

37 kDa

CAS编号和信息

9002-04-4

酶学委员会编号

EC 3.4.21.5

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from 50mM Sodium Citrate, 0.2M NaCl, 0.1% PEG-8000, pH 6.5.

浓度

来源于人血浆; ≥2700 NIH U/mg protein; 冻干前浓度：见COA

复溶

We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.

储存条件

2-8°C储存

运输条件

低温运输

稳定性与储存

长期储存2-8℃；复溶后建议分装，于-80℃冷冻保存。

高级数据

产品属性

Nacres	NA.54

质检证书(CoA,COO,BSE/TSE 和分析图谱)

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ26F0737104	分析证书	np330890
ZJ26F0737103	分析证书	np330890

技术文档和文章

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融合蛋白标签切除：常用蛋白酶的催化机制、识别特异性与选择策略

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