α-凝血酶

Name: α-凝血酶
SKU: np330890
Availability: InStock

CAS: 9002-04-4 货号: np330890 分子式: C₁₂H₁₀ClN₃S 分子量: 263.75 EC号: 232-648-7 PubChem CID: 65045

34篇引用文献 COA

有货

级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? 来源于人血浆; ≥2700 NIH U/mg protein; 冻干前浓度：见COA

Accession #

P00734

生物活性

≥2700 NIH U/mg protein

规格

库存

价格

数量

50U

np330890-50U

现货

¥1,699.90

250U

np330890-250U

期货

¥4,799.90

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概述规格储存与运输技术文档高级数据产品属性质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 34 篇同行评审文献引用。

概述

The Human Thrombin was activated from homogeneous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. These activating enzymes were removed after activation. Complete activation was observed on SDS-PAGE. This activated enzyme has a minimum activity of 2,700 NIH units/mg when compared to NIH standard thrombin.

Extinction Coefficient (1%) = 18.3

The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.

Protein is for research use only; not for human use.

规格

产品名称

α-凝血酶

别名

凝血因子 Ⅱa

英文别名

规格或纯度

生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, 来源于人血浆; ≥2700 NIH U/mg protein; 冻干前浓度：见COA

生化机理

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Activity regulation: Activity is promoted in the presence of negatively charged surfaces, such as polyphosphate and dextran sulfate. Inhibited by SERPINA5. Post-translational: The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). In the penultimate step of the coagulation cascade, prothrombin is converted to thrombin by the prothrombinase complex composed of factor Xa (F10), cofactor Va (F5), and phospholipids. This activation requires factor Xa-catalyzed sequential cleavage at 2 sites, Arg-314 and Arg-363, along 2 possible pathways. In the first pathway, the first cleavage occurs at Arg-314, leading to the formation of the inactive intermediate prethrombin-2. This pathway preferentially occurs on platelets and in the absence of cofactor Va. In the second pathway, the first cleavage occurs at Arg-363, which separates protease domain into 2 chains that remain connected through a disulfide bond and generates the active intermediate meizothrombin. The presence of cofactor Va directs activation along the meizothrombin pathway and greatly accelerates the rate of cleavage at Arg-363, but has a smaller effect on the cleavage of meizothrombin at Arg-314. Meizothrombin accumulates as an intermediate when prothrombinase is assembled on the membrane of red blood cells.

生物活性

≥2700 NIH U/mg protein

种属

人(Human)

来源

天然

预测分子量

37 kDa

CAS编号和信息

9002-04-4

酶学委员会编号

EC 3.4.21.5

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from 50mM Sodium Citrate, 0.2M NaCl, 0.1% PEG-8000, pH 6.5.

浓度

来源于人血浆; ≥2700 NIH U/mg protein; 冻干前浓度：见COA

复溶

We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.

储存条件

2-8°C储存

运输条件

低温运输

稳定性与储存

长期储存2-8℃；复溶后建议分装，于-80℃冷冻保存。

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

产品属性

Nacres	NA.54

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ26F0737104	分析证书	np330890
ZJ26F0737103	分析证书	np330890

技术文档和文章

凝血与抗凝蛋白网络在心血管疾病中的动态重构与研究体系

目录：技术文章

探索主题:

心血管疾病凝血酶纤溶凝血抗凝组织因子蛋白C 抗凝血酶组织因子途径抑制物血管性血友病因子

Dynamic Remodeling of the Coagulation-Anticoagulation Protein Network in Cardiovascular Diseases and Its Research Framework

目录：技术文章

探索主题:

Cardiovascular diseases Thrombin Fibrinolysis Coagulation Anticoagulation Tissue Factor Protein C Antithrombin Tissue Factor Pathway Inhibitor Von Willebrand Factor

纤溶系统激活因子的作用机制、功能差异与溶栓应用

目录：技术文章

探索主题:

纤维蛋白纤溶酶纤溶酶原尿激酶链激酶纤溶系统组织型纤溶酶原激活剂 tPA 溶栓出血风险

Mechanisms of Action, Functional Differences, and Thrombolytic Applications of Fibrinolytic System Activators

目录：技术文章

探索主题:

fibrin Plasmin Thrombolysis Plasminogen Urokinase Streptokinase tPA Fibrinolytic System Tissue-Type Plasminogen Activator Bleeding Risk

冰冻组织切片制备及切片冻存操作规程

目录：实验方案（Protocols）

探索主题:

免疫组织化学免疫荧光脂质染色酶组织化学

Standard Operating Procedure for Frozen Tissue Section Preparation and Section Storage

目录：实验方案（Protocols）

探索主题:

Immunohistochemistry lipid staining Enzyme Histochemistry

内肽酶与外肽酶的功能差异及其在疾病机制研究中的应用

目录：技术文章

探索主题:

蛋白质降解 Endopeptidase 氨基肽酶外肽酶 exopeptidase 内肽酶肽段加工羧肽酶二肽基肽酶

Functional Differences Between Endopeptidases and Exopeptidases and Their Applications in Disease Mechanism Research

目录：技术文章

探索主题:

Protein degradation Endopeptidase aminopeptidase exopeptidase Peptide Processing carboxypeptidase dipeptidyl peptidase

融合蛋白标签切除：常用蛋白酶的催化机制、识别特异性与选择策略

目录：技术文章

探索主题:

凝血酶融合蛋白标签切除 TEV蛋白酶 HRV 3C蛋白酶肠激酶 Factor Xa SUMO蛋白酶识别序列重组蛋白纯化

Fusion-Protein Tag Removal: Catalytic Mechanisms, Recognition Specificity, and Selection Strategies of Common Proteases

目录：技术文章

探索主题:

Thrombin Factor Xa fusion protein tag removal TEV protease HRV 3C protease enterokinase SUMO protease recognition sequence recombinant protein purification

纤连蛋白的结构与功能：细胞黏附、皮肤创伤修复与外用应用

目录：技术文章

探索主题:

纤连蛋白 FN

Structure and Functions of Fibronectin: Cell Adhesion, Cutaneous Wound Repair, and Topical Applications

目录：技术文章

探索主题:

fibronectin FN

丝氨酸内切蛋白酶的催化机制、分类特征与实验应用

目录：技术文章

探索主题:

胰蛋白酶丝氨酸蛋白酶凝血酶催化三联体蛋白水解丝氨酸内切蛋白酶 EC 3.4.21 枯草杆菌蛋白酶